Comparative Studies on Microbioassay and Chemical Procedure for Quantitative Determination of Niacin in Feed Additives

사료첨가제중(飼料添加劑中) Niacin의 미생물학적정량법(微生物學的定量法)과 화학적정량법(化學的定量法)의 비교시험(比較試驗)

  • Cho, Jong-Hoo (Institute of Veterinary Research, Office of Rural Deelpment) ;
  • Hwang, Dae-Woo (Institute of Veterinary Research, Office of Rural Deelpment) ;
  • Han, Suu-Nam (Department of Veterinary Medicine, College of Agriculture. Seoul National University)
  • Published : 1974.03.30

Abstract

Mohan's aniline procedure for the quantitative determination of niacin in feed additives containing various vitamines and minerals was compared with microbiological procedure utilizing growth rate of Lactobacillus arabinosus 17-5. Microbioassay was more sensitive than chemical procedure to the detection of standard niacin and was applicable to the determination of minute amounts of niacin. while both microbioassay and chemical proce dure were discovered to be unsatisfactory by the recovery test for the determination of niacin in feed additives containing much interfering substances. But the possibility of the determination of niacin in feed additives on chemical prccedure together with microbioassay was proved.

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