Korean Journal of Microbiology (미생물학회지)
- Volume 10 Issue 2
- /
- Pages.69-72
- /
- 1972
- /
- 0440-2413(pISSN)
- /
- 2383-9902(eISSN)
Crytallization of $\alpha$ -amylase and protease of ASP. oryzae from Column Chromatograph
컬럼 크로마토그라피에 의한 아스퍼질러스 계통의 $\alpha$ -아미라제 및 프로테아제의 결정화 2
Abstract
The studies of neutral protease which was obtained by passing through Sephadex A-50 had been reported not long ago. Since that time the author also conducted the research to be investigated the physical properties of acid protease absorbed by Sephadex A-50. The results are summarized as follows : 1) Cultivating Aspergillus oryza SHW-131 on a wheat bran medium, the acid protease including neutral protease is very sensitive for temperature. 3) Activity of acid protease is very sensitive for temeprature. 3) This enzyme was proved, what is called, to be a sort of weak acid protease. It's optimum pH was lied in about 4.5. 4) A range of pH for stability is far more narrow than any other protease. 5) The acid protease is dropped by EDTA solution in its activity.
Keywords