Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Studies on Enzyme of the Thermophilic Mold-Part. 3. Thermophilic mold amylase-

고온성 사상균의 효소에 관한 연구-(제3보) 고온성 사상균의 Amylase-

  • Chung, Dong-Hyo (Dept. of Fermentation Technology, College of Engineering Kon-Kuk University) ;
  • Lee, Ke-Ho (College of Agriculture, Seoul National University)
  • 정동효 (건국대학교 공과대학 발효공학과) ;
  • 이계호 (서울대학교 농과대학)
  • Published : 1970.12.30
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Abstract

1. Purification of amylase system produced from Humicola sp. by a submerged culture eras carried out. 2. By DEAE-Cellulose column chromatography amylase system was separated into two fractions eluted at 0.05M and 0.5M phosphate buffer solution of pH 6.0. 3. The saccharogenic amylase was mostly composed of. the fraction of 0.05M phosphate buffer solution of pH 6.0 while the dextrinogenic amylase was perseted in fraction of 0.5M phosphate buffer solution of pH 6.0 4. It was found that the optimum pH of this saccharogenic amylase was within the range of from 4.5 to 5.5, stable pH was within the range of from 4.0 to 9.0 and optimum temperature was $60-65^{\circ}C$. This amylase was stable at $70^{\circ}C$ for ten minutes but completely inactivated $80^{\circ}C$ above.

1. 내열성 사상균 Humicola속의 Amylase를 정제하였다. 2. DEAE-Cellulose Column Chromatography로 2개의 활성구분으로 나누어 졌다. 3. 즉 pH 6.0, 0.05M와 0.5M의 인산염완충용액으로 용출시킬때 전자에서 당화형, 후자에서 호정화형 Amylase가 각각 분리되었다. 4. 이 당화형 Amylase의 최적 pH는 $4.5{\sim}5.5$이고, 안정 pH 범위는 $4.0{\sim}9.0$범위였다. 최적온도는 $60{\sim}65^{\circ}C$로 다른 사상균의 그것보다 훨씬높고 $80^{\circ}C$ 이상에서는 불활성화 되었다.

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