Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Studies on Enzyme of the Thermophilic Mold-(Part 2.) Thermophilic mold alkaline protease-

고온성 사상균의 효소에 관한 연구-(제2보) 고온성사상균의 알카리성 protease-

  • Chung, Dong-Hyo (Dept. of Fermentation Technology, Kon-Kuk University) ;
  • Lee, Ke-Ho (College of Agriculture, Seoul National University)
  • 정동효 (건국대학교 공과대학 발효공학과) ;
  • 이계호 (서울대학교 농과대학)
  • Published : 1970.12.30
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Abstract

1. The preparation and some enzymatic properties of crude alkaline protease from a thermophilic mold, Myriococcum sp. was investigated. 2. Optimum pH for the hydrolysis of casein was 9.0 at $50^{\circ}C$ for 10 minutes. Optimum temperature was $55^{\circ}C$ at pH 9.0 for 10 minutes. The enzyme was highly stable at the range of pH 6.0 to 11.0 at $30^{\circ}C$ 3. The alkaline protease in the culture filterate was isolated two fractions by elution column chromatography on DEAE-Cellulose.

(1) 고온성사상균인 Myriococcum 속의 알칼리성 protense의 생성과 조효소의 성질을 검토하였다. (2) 조호소의 최적 pH는 9.0이고, 최적 온도는 $55^{\circ}C$였다. 그리고 안정 pH 범위는 6.0에서 11.0이었다. (3) 조효소용액을 DEAE-Cellulose column Chromatography로 2개의 다른 활성부분으로 나누어졌다.

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