Applied Biological Chemistry

  • Volume 11
  • /
  • Pages.55-61
  • /
  • 1969
  • /
  • 2468-0834(pISSN)
  • /
  • 2468-0842(eISSN)
The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Studies on the Glucose Isomerizing Enzyme -Part III The Effects of Metal Ions on the Reaction of K-17 Strain's Glucose Isomerizing Enzyme-

포도당(葡萄糖) 이성화(異性化) 효소(效素)에 관(關)한 연구(硏究) -제3보(第三報) 분리(分離) 선정(選定)된 균주(菌株) 효소(酵素)의 작용(作用)에 미치는 금속염(金屬鹽)의 영향(影響)에 대(對)해서-

  • Seu, J.H. (Dept. of Applied Microbiology, Agr. College, Kyung-Pook National University) ;
  • Kim, C.K. (Dept. of Applied Microbiology, Agr. College, Kyung-Pook National University) ;
  • Ki, W.K. (Dept. of Applied Microbiology, Agr. College, Kyung-Pook National University) ;
  • Rhee, I.K. (Dept. of Applied Microbiology, Agr. College, Kyung-Pook National University) ;
  • Kwon, T.J. (Dept. of Applied Microbiology, Agr. College, Kyung-Pook National University) ;
  • Woo, D.L. (Dept. of Applied Microbiology, Agr. College, Kyung-Pook National University)
  • Published : 1969.04.30
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Abstract

This K-17 strain was not absolutely requiring xylose as an inducer for enzyme formations. The most activity of this enzyme was lost when treated at $75^{\circ}C$. for 30 hours but was not influenced at $70^{\circ}C$. for 70 hours of treatment. The activity of this enzyme was increased by the addition of magnesium ions or cobalt ions in the reaction system. In the studies, we found that the magnesium ions simply activate the enzyme reaction and the cobalt ions do not but protect the enzyme from heat inactvation. And it was also found the phosphate buffer solution was very suitable as glucose dissolving solvent on the enzyme reaction. The mixed carbon source medium containing glucose, fructose, sorbitol, xylose and sucrose was more favorable for enzyme production then a sole carbon source containing medium on the shaking culture method.

본(本) 연구(硏究)에서 사용(使用)한 K-17 균주(菌株)의 효소생성(酵素生成)및 그의 금속염(金屬鹽) 등(等)에 대(對)한 영향(影響)등(等)을 요약(要約)하면 대략(大略) 다음과 같다. 1. 본(本) 균주(菌株)는 Glucose Isomerizing Enzyme 생성(生成)에 있어서 Xylose를 절대적(絶對的)으로 요구(要求)하지 않는다. 2. 본(本) 효소(酵素)는 Free Incubation 상태(狀態)에서는 $75^{\circ}C$, 30시간(時間)의 가열(加熱)로서 대부분(大部分)의 효소(酵素)가 실활(失活)된다. 3. 금속(金屬) Ion 중(中) $Mg^{++}$는 효소작용(酵素作用)에 있어 일종(一種) 의 Activator로 작용(作用)하며 $Co^{++}$는 Activator로 서의 작용(作用)은 없고 효소(酵素)의 열(熱) 실활(失活)을 보호(保護)하는 작용(作用)을 나타낸다. 4. 본(本) 효소작용(酵素作用)에 있어서 Glucose의 용매(溶媒)로서는 Phosphate Buffer가 가장 우수(優秀)한 결과(結果)를 나타내었다. 5. 본(本) 균주(菌株)의 효소(酵素) 생성(生成) 배지(培地)로서는 탄소원(炭素源)으로서 주어지는 당류(糖類)의 혼용(混用)이 단용(單用)에 비(比)해 균체(菌體)의 수량(收量)이나 효소능(酵素能)이 다같이 높아짐을 알게 되었다.

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