Proceedings of the Plant Resources Society of Korea Conference (한국자원식물학회:학술대회논문집)

The Plant Resources Society of Korea (한국자원식물학회)
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Baicalein induces cell death in Human Lung Carcinoma A549 Cells: Role of Apoptosis and Autophagy pathway

인체폐암 A549 세포에서 Baicalein에 의한 세포사멸 유도: Apoptosis와 Autophagy 경로의 역할

  • Kim, Chul Hwan (Animal & Plant Utilization Team, Nakdonggang National Institute of Biological Resources) ;
  • Hwang, Buyng Su (Animal & Plant Utilization Team, Nakdonggang National Institute of Biological Resources) ;
  • Jeong, Yong Tae (Animal & Plant Utilization Team, Nakdonggang National Institute of Biological Resources) ;
  • Kim, Min-Jin (Animal & Plant Utilization Team, Nakdonggang National Institute of Biological Resources) ;
  • Shin, Su Young (Animal & Plant Utilization Team, Nakdonggang National Institute of Biological Resources) ;
  • Oh, Young Taek (Animal & Plant Utilization Team, Nakdonggang National Institute of Biological Resources) ;
  • Eom, Jung Hye (Animal & Plant Utilization Team, Nakdonggang National Institute of Biological Resources) ;
  • Lee, Seung Young (Performance Management Division, Nakdonggang National Institute of Biological Resources) ;
  • Choi, Kyung Min (Performance Management Division, Nakdonggang National Institute of Biological Resources) ;
  • Cho, Pyo Yun (Animal & Plant Utilization Team, Nakdonggang National Institute of Biological Resources) ;
  • Jeong, Jin-Woo (Animal & Plant Utilization Team, Nakdonggang National Institute of Biological Resources)
  • 김철환 (국립낙동강생물자원관 동식물활용연구팀) ;
  • 황병수 (국립낙동강생물자원관 동식물활용연구팀) ;
  • 정용태 (국립낙동강생물자원관 동식물활용연구팀) ;
  • 김민진 (국립낙동강생물자원관 동식물활용연구팀) ;
  • 신수영 (국립낙동강생물자원관 동식물활용연구팀) ;
  • 오영택 (국립낙동강생물자원관 동식물활용연구팀) ;
  • 엄정혜 (국립낙동강생물자원관 동식물활용연구팀) ;
  • 이승영 (국립낙동강생물자원관 혁신성과부) ;
  • 최경민 (국립낙동강생물자원관 혁신성과부) ;
  • 조표연 (국립낙동강생물자원관 동식물활용연구팀) ;
  • 정진우 (국립낙동강생물자원관 동식물활용연구팀)
  • Published : 2019.04.25
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Abstract

Baicalein is one of the main flavonoids derived from roots of Scutellaria baicalensis Georgi, a traditional Oriental medicine. Although baicalein has high antitumor effect on several human carcinomas, the mechanism responsible for this property is not unclear. In this study, the data revealed that baicale-ininduced growth inhibition was associated with the induction of apoptosis connecting with cytochrome c release, down-regulation of anti-apoptotic Bcl-xl and increased the percentage of cells with a loss of mitochondria membrane permeabilization. Baicalein also induced the proteolytic activation of caspases and cleavage of PARP; however, blockage of caspases activation by z-VAD-fmk inhibited baicalein-induced apoptosis. In addition, baicalein enhanced the formation of autophagosomes and up-regulated LC3-II/LC3-I ratio. Interestingly, the pretreatment of bafilomycin A1 recovered baicalein-induced cell death suggesting that autophagy by baicalein roles as protective autophagy. Taken together, our results indicated that this flavonoid induces apoptosis and cell protective autophagy. These data means combination treatment with baicalein and autophagy inhibitor might be a promising anticancer drug.

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