한국정보통신학회:학술대회논문집 (Proceedings of the Korean Institute of Information and Commucation Sciences Conference)

한국정보통신학회 (The Korea Institute of Information and Commucation Engineering)
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Semliki forest virus 감염에 의한 뉴우런의 탈수초

Demyelination of Neuron by Infection of Semliki Forest Virus

  • Kim, Hyun Joo (Department of Anesthesiology and Pain Medicine, Yonsei University College of Medicine) ;
  • Kim, Ji-Young (Department of Anesthesiology and Pain Medicine, Yonsei University College of Medicine) ;
  • Sa, Young-Hee (Department of Anesthesiology and Pain Medicine, Yonsei University College of Medicine) ;
  • Hong, Seong-Karp (Division of Bio and Health Sciences, Mokwon University)
  • 발행 : 2016.05.25
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초록

쥐의 배아의 척수신경절로 부터 슈반세포와 뉴런 세포가 각각 배양되었다. in vitro 시스템에서 배양되었다. 항 유사분열제로 처리한 정제 뉴런 세포와 정제 슈반세포들이 공동배양 되었고 그 때 수초화 과정이 진행되었다. 수초화된 공동 배양에 Semliki forest virus를 감염시켜 탈수초화를 진행 시켰다. 우리는 수초화의 형성을 의미하는 neuropeptide Y의 항체를 이용하여 수초화와 탈수초화를 확인하였다.

Schwann cells and neuron cells from dorsal root ganglion (DRG) in embryos of rat were cultured in vitro respectively. The purified neronal cells added with anti-mitotic agents and purified Schwann cells were cocultured and then accomplished myelination processing. Infection of Semliki forest virus into this myelinated co-culture system was performed and then accomplished demyelination. We identified myelination and demyelination processing using antibody of neuropeptide Y meaning presence of myelinated neuron.

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