Proceedings of the Korean Institute of Information and Commucation Sciences Conference (한국정보통신학회:학술대회논문집)

The Korea Institute of Information and Commucation Engineering (한국정보통신학회)
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Demyelination of Neuron by Infection of Semliki Forest Virus

Semliki forest virus 감염에 의한 뉴우런의 탈수초

  • Kim, Hyun Joo (Department of Anesthesiology and Pain Medicine, Yonsei University College of Medicine) ;
  • Kim, Ji-Young (Department of Anesthesiology and Pain Medicine, Yonsei University College of Medicine) ;
  • Sa, Young-Hee (Department of Anesthesiology and Pain Medicine, Yonsei University College of Medicine) ;
  • Hong, Seong-Karp (Division of Bio and Health Sciences, Mokwon University)
  • Published : 2016.05.25
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Abstract

Schwann cells and neuron cells from dorsal root ganglion (DRG) in embryos of rat were cultured in vitro respectively. The purified neronal cells added with anti-mitotic agents and purified Schwann cells were cocultured and then accomplished myelination processing. Infection of Semliki forest virus into this myelinated co-culture system was performed and then accomplished demyelination. We identified myelination and demyelination processing using antibody of neuropeptide Y meaning presence of myelinated neuron.

쥐의 배아의 척수신경절로 부터 슈반세포와 뉴런 세포가 각각 배양되었다. in vitro 시스템에서 배양되었다. 항 유사분열제로 처리한 정제 뉴런 세포와 정제 슈반세포들이 공동배양 되었고 그 때 수초화 과정이 진행되었다. 수초화된 공동 배양에 Semliki forest virus를 감염시켜 탈수초화를 진행 시켰다. 우리는 수초화의 형성을 의미하는 neuropeptide Y의 항체를 이용하여 수초화와 탈수초화를 확인하였다.

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