Proceedings of the KAIS Fall Conference (한국산학기술학회:학술대회논문집)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Antioxidant activity of extracts from Blueberry

블루베리 추출물의 항산화 활성

  • O, Jae-Yeong (School of Marine Biomedical Science, Jeju National University) ;
  • Kang, Na-Lae (School of Marine Biomedical Science, Jeju National University) ;
  • Kang, Seong-Woong (School of Marine Biomedical Science, Jeju National University) ;
  • Song, Hyung-Yong (School of Marine Biomedical Science, Jeju National University) ;
  • Kim, Hyeon-A (Dept. of Chemistry, Jeju National University) ;
  • Hwang, Eun-Yeong (Dept. of Chemistry, Jeju National University) ;
  • Jeon, You-Jin (School of Marine Biomedical Science, Jeju National University)
  • 오재영 (제주대학교 해양의생명과학부) ;
  • 강나래 (제주대학교 해양의생명과학부) ;
  • 강성웅 (제주대학교 해양의생명과학부) ;
  • 송현용 (제주대학교 해양의생명과학부) ;
  • 김현아 (제주대학교 화학과) ;
  • 황은영 (제주대학교 화학과) ;
  • 전유진 (제주대학교 해양의생명과학부)
  • Published : 2010.11.12
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Abstract

In this study, antioxidant activity of enzymatic, ethanolic and aqueous extract from Blueberry were evaluated by measuring the scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH). Enzymatic extract were prepared by enzymatic hydrolysis of Blueberry using food grade five different carbohydrases (Viscozyme, celluclast, AMG, Termarmyl, Ultraflo) and five proteases (Protamex, Kojizyme, Neutrase, Flavourzyme, Alcalase). The ethanol extract were lower than enzymatic extracts in yield, but higher in ployphenolic contents. The 70% ethanolic extract of Blueberry exhibited better DPPH radical scavenging activity compared to those of other extracts. These results suggest that Blueberry would be a good raw materials for antioxidant.

본 연구에서는 제주산 블루베리의 항산화활성을 알아보기 위하여 블루베리로부터 에탄올과 효소적 가수분해를 이용하여 각각의 추출물을 제조한 후 이들로부터 활성산소종의 소거활성을 측정하였다. 여기에서 효소 추출물보다 에탄올 추출물이 추출수율은 낮았으나, 폴리페놀 함량과 DPPH radical 소거활성이 모두 높게 나타났다. 특히 70% 에탄올 추출물은 효소추출물보다 폴리페놀 함량은 1000 mg 이상 높게 나타났으며, DPPH radical 소거활성은 125 ${\mu}g$/mL sample로 측정하였을 때에 크게는 53%작게는 27%높게 나타났다.

Keywords