Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference (한국전기전자재료학회:학술대회논문집)
- 2008.06a
- /
- Pages.512-513
- /
- 2008
The effect of 100KHz PWM LED light irradiation on RAT bone-marrow cells
100kHz PWM LED 광조사가 백서 골수세포에 미치는 영향
- Cheon, Min-Woo (Chosun Univ.) ;
-
Kim, Seong-Hwan
(Chosun Univ.)
;
- Kim, Young-Pyo (Dongshin Univ.) ;
-
Lee, Ho-Sic
(Dongshin Univ.)
;
- Park, Yong-Pil (Dongshin Univ.) ;
-
Yu, Seong-Mi
(Gwangju Health College)
;
- Lee, Hee-Gap (KCCI) ;
- Kim, Tae-Gon (Bioateco Inc.)
- 천민우 (조선대학교) ;
-
김성환
(조선대학교)
;
- 김영표 (동신대학교) ;
-
이호식
(동신대학교)
;
- 박용필 (동신대학교) ;
-
유성미
(광주보건대학교)
;
- 이희갑 (대한상공회의소) ;
- 김태곤 ((주)바이오아테코)
- Published : 2008.06.19
Abstract
The study examined what effects 100kHz PWM LED light irradiation causes to bone marrow cells of SD-Rat when LED characterized cheap and safe is used onto the light therapy by replacing the low 1evel laser. We developed the equipment palpating cell proliferation using a high brightness LED. This equipment was fabricated using a micro-controller and a high brightness LED, and designed to enable us to control light irradiation time, intensity, frequency and so on. Especially, to control the light irradiation frequency, FPGA was used, and to control the change of output value, TLC5941 was used. Consequent1y, the current value could be controlled by the change of 1eve1 in Continue Wave(CW) and Pulse Width Modulation(PWM), and the output of a high brightness LED could be controlled stage by stage. MTT assay method was chosen to verify the cell increase of two groups and the effect of irradiation on cell proliferation was examined by measuring 590nm transmittance of ELISA reader. As a result, the cell increase of Rat bone marrow cells was verified in 100kHz PWM LED light irradiation group as compared to non-irradiation group.