한국유가공학회:학술대회논문집
- 한국유가공기술과힉회 2007년도 추계학술발표대회
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- Pages.13-20
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- 2007
미생물을 이용한 우유 유래 항균펩타이드(락토페리신)의 생산
Production of milk-originated antimicrobial peptide, lactoferricin, in E. coli
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강대경
(단국대학교 동물자원학과)
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Kang, Dae-Kyung
(Department of Animal Resources Science, School of Bio-Resources Science, Dankook University)
- 발행 : 2007.09.14
초록
Bovine lactoferricin(LFcin B) is a peptide of 25 amino acids that originated from the N terminus of bovine lactoferrin, and is characterized as having potent antimicrobial activity against bacteria, fungi, protozoa and viruses. But, direct expression of Lfcin B is lethal to Escherichia coli. For the efficient production of Lfcin B in E. coli, we developed an expression system in which the gene for cationic Lfcin B was fused to an anionic peptide gene, and successfully expressed the concatemeric fusion gene in E. coli. The purified recombinant Lfcin B was found to have antimicrobial activity, as the native Lfcin B peptide does.