Proceedings of the Korean Society of Precision Engineering Conference (한국정밀공학회:학술대회논문집)
- 2005.10a
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- Pages.69-72
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- 2005
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- 2005-8446(pISSN)
Effects of Intermittent Hydrostatic Pressure on the Cell Adhesive force on the substrate
간헐적 정수압이 세포의 부착에 미치는 영향성 평가
- Kim Young-Jick (Dept. of Dental Lab. Science, Catholic Univ. of Pusan) ;
- Lee Myung-Kon (Dept. of Dental Lab. Science, Catholic Univ. of Pusan) ;
- Park Su-A (Dept. of Biomedical Eng. Inje Univ.) ;
- Shin Ho-Joon (Dept. of Biomedical Eng. Inje Univ.) ;
- Kim In-Ae (Dept. of Biomedical Eng. Inje Univ.) ;
- Lee Yong-Jae (Dept. of Biomedical Eng. Inje Univ.) ;
- Shin Ji-Won (Dept. of Biomedical Eng. Inje Univ.) ;
- Shin Jung-Woog (Dept. of Biomedical Eng. Inje Univ.)
- 김영직 (부산가톨릭대 치기공학과) ;
- 이명곤 (부산가톨릭대 치기공학과) ;
- 박수아 (인제대 의용공학과) ;
- 신호준 (인제대 의용공학과) ;
- 김인애 (인제대 의용공학과) ;
- 이용재 (인제대 의용공학과) ;
- 신지원 (인제대 의용공학과) ;
- 신정욱 (인제대 의용공학과)
- Published : 2005.10.01
Abstract
In this study, effects of IHPs with various resting times to cell adhesion were investigated through the measurements of cell adhesive force, number and area of focal contacts (stained vinculin spots), and projected cell area, perimeter and circularity. In addition correlation tests and curve estimations using the experimental results were performed fur the finding an essential factor for increment of cell adhesive force. Tn the results, immediately after mechanical stimuli (150 minutes after seeding) and one hour later (210 minutes after seeding), the average adhesive force of experimental group 5 (resting time: 15min) compared with that of control group at same culture time was increased significantly (p<0.05). Average projected area and perimeter of cells at Group 5 were increased significantly (p<0.05), while average circularity of cells at Group 5 incubated fur 210 minutes was decreased significantly (p<0.05). In the digital image analysis of focal contacts containing vinculins, area and numbers of focal contacts per cell at Group 5 were higher than those of the other groups. This study indicated that IHP with appropriate resting time could contribute in improving cell adhesive force, cell spreading, development of cytoskeleton and formation of focal contacts. And cell adhesive force was correlated to the morphological aspects of cell and development of focal contacts. Particularly, area of focal contacts was closely related to cell adhesive force.