Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)
- 2004.06a
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- Pages.229-229
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- 2004
Identification of Differentially Regulated Genes in Bovine Blastocysts using an Annealing Control Primer System
- Park, Sae-Young (Maria Infertility Hospital Medical Institute/Maria Biotech) ;
- Hwang, Kyu-Chan (Department of Animal Science, Chungbuk National University) ;
- Cui, Xiang-Shun (Department of Animal Science, Chungbuk National University) ;
- Shin, Mi-Ra (Department of Animal Science, Chungbuk National University) ;
- Kim, Eun-Young (Maria Infertility Hospital Medical Institute/Maria Biotec) ;
- Lee, Won-Don (Maria Infertility Hospita) ;
- Kim, Nam-Hyung (Department of Animal Science, Chungbuk National University) ;
-
Park, Sepill
(Maria Infertility Hospital Medical Institute/Maria Biotec)
;
- Lim, Jin-Ho (Maria Infertility Hospital)
- Published : 2004.06.01
Abstract
The identification of embryo-specific genes would provide insights into early embryonic development. However, the current methods employed to identify the genes that are expressed at a specific developmental stage are labor intensive and suffer from high rates of false positives. Here we employed a new and accurate reverse transcription-polymerase chain reaction (RT-PCR) technology that involves annealing control primers (ACPs) to identify the genes that are specifically or prominently expressed in bovine early blastocysts and hatched blastocysts produced in vitro. (omitted)