Abstract
Cultivation environment for effective production of shrimp in shrimp aquaculture, Institute of Marine & Fishery, Soonchunhyang University located at Tae-An, Chung-Nam Province, with the ultimate aim of probiotics development, was monitored. Several environmental factors including dissolved oxygen, water body temperature, pH, salinity, $NH_4-N$, $NO_2-N$, $NO_3-N$, chlorophyll a affecting on the growth of shrimp were examined. Concentrations of $NH_4-N$, $NO_2-N$, and $NO_3-N$ dissolved in water samples were determined to 0.024-0.034 mg/L, 0.03-0.02, and 0.004-0.009 mg/L, respectively. Chlorophyll a content was examined in the range of 0.002-0.118 $ug/m^3$. In order to understand the distribution of different bacteria in water samples collected in shrimp aquaculture, bacteria were isolated and enumerated on the marine agar plates. Total number of bacteria were increased to approximately $6.5\;{\times}\;10^4$. Thirteen predomonant bacteria were isolated and identified. As the results of BIOLOG test of the isolates, these bacteria were identified as Corynebacterium nirilophilus, Clavibacter agropyri, Sphingomonas adhaesiva, Brevundimonas vesicularis, Vibrio parahaemolyticus, Pseudomonas bathycetes, Vibrio tubiashii, Sphingomonas macrogoltabidus, Rhodococcus, rhodochrous, Burkholderia glumae, Corynebacterium urealyticus, Rhodococcus fascians, Psychrobacter immobilis, respectively. Further work will stimulate the elucidation of pathogenicity, corresponding bacteria related to environment and probiotics, providing good information for effective production of shrimp.
충남 태안소재 순천향대학교 해양연구소의 해양수산연구소에서 probiotics 개발을 궁극적인 목표로 하여 대하양식장에서 대하의 효율적 생산을 위한 생육환경을 조사하였다. 대하의 생장에 영향을 미치는 용존산소, 수체온도, pH, 염도, $NH_4-N$, $NO_2-N$, $NO_3-N$, 클로로필 a를 포함하는 몇가지 환경요인들을 조사하였다. 수 표본에 용존되어 있는 $NH_4-N$, $NO_2-N$, 그리고 $NO_3-N$의 농도는 각각 0.024-0.034 mg/L, 0.03-0.02, 0.004-0.009 mg/L였다. 클로로필 a는 0.002-0.018 $ug/m^3$ 범위에 있었다. 양식장에서 채취한 수 표본에서 다른 세균들의 분포를 알아보기 위하여 marine agar plates에서 분리 계수하였다. 총세균수는 대략 $6.5\;{\times}\;10^4$까지 증가하였다. BIOLOG 시험들 통하여 13가지의 분리 세균들은 Corynebacterium nirilophilus, Clavibacter agropyri, Sphingomonas adhaesiva, Brevundimonas vesicularis, Vibrio parahaemolyticus, Pseudomonas bathycetes, Vibrio tubiashii, Sphingomonas macrogoltabidus, Rhodococcus, rhodochrous, Burkholderia glumae, Corynebacterium urealyticus, Rhodococcus fascians, Psychrobacter immobilis로 각각 동정되었다. 향후 연구는 대하의 효율적인 생산을 위한 정보를 제공하기 위한 병독성이나 환경과 probiotics의 연관성을 규명에 집중 될 것이다.
(Department of Life Science, Soonchunhyang University)
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