한국생물공학회:학술대회논문집

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Analysis for the function of the N-terminal repeat region of Bacillus polymyxa CFTase

  • Kim, Byoung-Woo (Department of Life Science and Biotechnology, Dongeui University) ;
  • Park, Jung-Ha (Department of Life Science and Biotechnology, Dongeui University) ;
  • Kim, Eun-Young (Department of Life Science and Biotechnology, Dongeui University) ;
  • Kim, Kwang-Hyoun (Department of Life Science and Biotechnology, Dongeui University) ;
  • Kwon, Hyoun-Ju (Department of Life Science and Biotechnology, Dongeui University)
  • Published : 2003.10.22
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Abstract

Previously we reported the cloning and sequence analysis of a CFTase gene from Bacillus polymyxa. CFTase was divided into five distinct regions. In order to understand a role of the N-terminal repeat region on the function of CFTase from Bacillus polymyxa MGL21, deletion mutantCFTase ${\Delta}N$ was prepered. Recombinant protein was overproduced in E. coli as inclusion body, solubilized in bufer containing 8M urea, and refolded in the phosphate buffer. The molecular weight of the purified wild type CFTase and CFTase ${\Delta}N$ were 148kDa , 108kDa, respectively.

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