한국생물공학회:학술대회논문집

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Analysis for the function of the core region of Bacillus polymyxa CFTase

  • Kwon, Hyun-Ju (Department of Life Science and Biotechnology, Dongeui University) ;
  • You, Kyung-Ok (Department of Life Science and Biotechnology, Dongeui University) ;
  • Park, Ju-Hee (Department of Life Science and Biotechnology, Dongeui University) ;
  • Oh, You-Na (Department of Life Science and Biotechnology, Dongeui University) ;
  • Kim, Kwang-Hyun (Department of Life Science and Biotechnology, Dongeui University) ;
  • Kim, Byung-Woo (Department of Life Science and Biotechnology, Dongeui University)
  • Published : 2003.10.22
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Abstract

Sequence analysis indicated that Bacillus polymyxa MGL21 CFTase was divided into five distinct regions. CFTase contained three regions of repeat sequences at the N-terminus and C-terminus. The endo-inulinase region of homology (ERH) of CFTase was similar to that of Pseudomonas mucidolens endo-inulinase. Furthemore, CFTase possessed a highly conserved core region. In order to understand the role of the core region on the function of CFTase from B. polymyxa MGL21 CFTase ${\Delta}NC$ was prepared. The molecular weight of the purified wild type CFTase and $CFTase{\Delta}NC$ were 148kDa, 90kDa, respectively.

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