Biosurfactant 생산균주 Pseudomonas aeruginosa F722의 배양특성

P. aeruginosa F722는 탄화수소를 분해하는 과정에 biosurfactant (BS)를 생산한다. 탄화수소 분해에 사용되는 C-배지에서 BS 생산량은 0.78 $g/{\ell}$ 이였나, 질소원과 탄소원을 각각 0.05% (w/v) $NH_4Cl\;+\;0.1%$ (w/v) yeast extract과 3.0% (w/v) glucose로 조정한 경우는 BS 생산량이 1.66 $g/{\ell}$로 증가하였다. 최적의 BS 생산조건으로 배양하는 동안 air 1.0 LPM를 공급해 주었을 때는 공기를 공급하지 않을 때의 1.66 $g/{\ell}$보다 약 20% 증가한 1.94 $g/{\ell}$이였다. 뿐만 아니라, glucose 분해속도는 대수증식기와 정지기에서 air를 공급하지 않은 경우0.25, 0.18 $h^{-1}$였으나, 1.0 LPM를 공급한 경우 0.33, 0.29 $h^{-1}$로 조사되었다. 또한, air를 공급하면서 BS 생산 실험을 수행하였을 때, BS 활성이 더 안정적이었다.

P. aeruginosa F722 produces biosurfactant (BS) while degrading hydrocarbons. BS production was 0.78 $g/{\ell}$ on the C-medium. However, BS production increased by 1.66 $g/{\ell}$ on the condition of 0.05% (w/v) $NH_4Cl+0.1%$ (w/v) yeast extract and 3.0% (w/v) glucose, which was proved to be advantageous to BS production. In the condition of aeration of 1.0 liter per minute (LPM), BS production was increased 20% (1.94 $g/{\ell}$)more than 1.66 $g/{\ell}$ produced when the air was not supplied. Moreover, the velocity of glucose degradation at both of log and stationary growth phases increased from 0.25 and 0.18 $h^{-1}$ to 0.33 and 0.29 $h^{-1}$ respectively when the air was supplied. Besides, BS activity was more stabilized on the condition of air supply.