High Performance Liquid Chromatographic Analysis of Isoflavones in KUNBO

  • Jung, Da-Young (Drug Research and Development Team, Korea Institute of Oriental Medicine) ;
  • Chang , Young-Eun (Drug Research and Development Team, Korea Institute of Oriental Medicin) ;
  • Ha , Hye-Kyung (Drug Research and Development Team, Korea Institute of Oriental Medicin) ;
  • Yang , Ha-Ru (Drug Research and Development Team, Korea Institute of Oriental Medicin) ;
  • Lee, Ho-Young (Drug Research and Development Team, Korea Institute of Oriental Medicin) ;
  • Kim, Chung-Sook (Drug Research and Development Team, Korea Institute of Oriental Medicine)
  • Published : 2003.10.01

Abstract

Phytoestrogen has been used as supplement in order to treat osteoporosis. The representative phytoestrogen, isoflavones, are daidzein, genistein and formononetin which were present highly in legumes. We have studied the quantitative analysis of isoflavones in KUNBO by HPLC. KUNBO, a mixed herbal extract including Astragali Radix and Rhynchosiae nulubilis Semen (Leguminosae), etc., is a nutraceutical candidate for type I osteoporosis. olumn used in HPLC was LUNA 5${\mu}$ C18 (250 ${\times}$4.6mm) (Phenmenex Co.m Torroance, CA, U.S.A.). (omitted)

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