Proceedings of the PSK Conference (대한약학회:학술대회논문집)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Platycodin D Induced NF-$textsc{k}$B Activation and Apoptosis in Immortalized Keratinocytes

  • Ahn, Kwang-Seok (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Hahn, Bum-Soo (Metabolic Engineering Division, National Institute of Agricultural Biotechnolog) ;
  • Lee, Eun-Bang (Metabolic Engineering Division, National Institute of Agricultural Biotechnolog) ;
  • Kim, Yeong-Shik (Metabolic Engineering Division, National Institute of Agricultural Biotechnology)
  • Published : 2003.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, we investigated the molecular pathways targeted by platycodin D, which could involve apoptosis in immortalized human keratinocytes (HaCaT). We demonstrated that platycodin D-mediated apoptosis of HaCaT cells exhibited representative features, including DNA fragmentation, caspase-3, caspase-8 activation, and upregulation of Fas and FasL expression, but not p53 activation. To investigate the events involved in activation-induced FasL upregulation, we have examined mRNA accumulation, protein expression, and NF-$\kappa$B activity to elucidate transcription level in the HaCaT cell line treated with platycodin D. (omitted)

Keywords