Proceedings of the PSK Conference (대한약학회:학술대회논문집)
- 2003.10b
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- Pages.151.3-152
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- 2003
Aromatic diamine JSH-21 inhibits LPS-induced NO production by targeting NF-kB signaling
- Shin, Hyun-Mo (College of Pharmacy, Chungbuk National University) ;
- Jung, Sang-Hun (College of Pharmacy, Chungnam National University) ;
- Min, Kyung-Rak (College of Pharmacy, Chungnam National University) ;
- Kim, Young-Soo (College of Pharmacy, Chungnam National University)
- Published : 2003.10.01
Abstract
Aromatic diamine JSH-21 showed an IC50 value of 9.2 uM with 74.5% inhibition at 30 uM, 53.5% at 10 uM and 24.5% at 3 uM on LPS-induced NO production in murine macrophages Raw 264.7. To examine whether inhibitory effect on NO production by JSH-21 was attributed to influence on iNOS expression, iNOS transcript and protein were analyzed by sequantitative RT-PCR and immunoblot analysis. Consistent with previous result on NO production, treatment of the Raw 264.7 cells with JSH-21 decreased the LPS-induced expression of iNOS transcript and protein in a dose-dependent manner with IC50 values of about 10 uM. (omitted)
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