Proceedings of the PSK Conference (대한약학회:학술대회논문집)
- 2003.10b
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- Pages.145.2-145.2
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- 2003
Dye-silver double staining method for proteins in SDS-polyacrylamide gels using a dye as a silver sensitizer
- Jin, Li-Tai (College of Pharmacy, Chonnam National University) ;
- Hwang, Sun-Young (College of Pharmacy, Chonnam National University) ;
- Yoo, Gyurng-Soo (College of Pharmacy, Chonnam National University) ;
- Choi, Jung-Kap (College of Pharmacy, Chonnam National University)
- Published : 2003.10.01
Abstract
We have developed a silver staining method using a dye as a silver sensitizer. Dye staining is performed in combination with silver nitrate staining. Dye-silver staining shortens the time of silver staining (~1 hr) and improves the sensitivity better than that of silver diamine stain (1-10 ng) or comparable to that of silver nitrate stain with glutaraldehyde as a silver sensitizer. In dye staining (silver sensitizing step), it has been proven that the sensitivity is at least 4 times comparing with that of CBBR stain and staining time is about 45 min. (omitted)
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