Proceedings of the PSK Conference (대한약학회:학술대회논문집)

The Pharmaceutical Society of Korea (대한약학회)
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In vitro culture of skin cells on a crosslinked gelatin based scaffold for artificial skin

  • Shin, In-Soo (Blood Products Division, Biologics Evaluation Department) ;
  • Kwon, Oh-Hee (Korea Food and Drug Administration, Biologics Evaluation Departmen) ;
  • Kim, Soon-Nam (Korea Food and Drug Administration, School of Chemical Engineering Hanyang University) ;
  • Hong, Choong-Man (Korea Food and Drug Administration, School of Chemical Engineering Hanyang University) ;
  • Lee, Ki-Hong (Korea Food and Drug Administration, School of Chemical Engineering Hanyang University) ;
  • Oh, Ho-Jung (Korea Food and Drug Administration, School of Chemical Engineering Hanyang University) ;
  • Yoo, Si-Hyung (Korea Food and Drug Administration, School of Chemical Engineering Hanyang University) ;
  • Lim, Jae-Hyun (Korea Food and Drug Administration, School of Chemical Engineering Hanyang University) ;
  • Choi, Seung-Eun (Korea Food and Drug Administration, School of Chemical Engineering Hanyang University)
  • Published : 2003.10.01
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Abstract

To satisfy the increasing medical demanding especially for sever burn patients to regenerate full thickness wound cure, this study developed dermis with gelatin based scaffold and perform the biocompatibility tests. To prepare scaffold 30% of gelatin was mixed with sieved salt and dried in the mold to shape then, cross linked with a water-soluble cross-linker, EDAC. Preparing the cell for seeding from a rabbit skin, the fibroblast and keratinocyte were successfully isolated and cultured in vitro. After cell and scaffold were ready, the fibroblast was seeded to the scaffold (∼10$\^$6/ cell/cm ) for preparing dermis and keratinocyte was cultured until forming the sheet. (omitted)

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