Proceedings of the PSK Conference (대한약학회:학술대회논문집)
- 2003.04a
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- Pages.300.2-300.2
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- 2003
Encapsulation of Plasmid DNA in Liposomes: Preparation and Characterization
- Park, Hyo-Min (College of Pharmacy, Ewha Womans University) ;
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Lee, Hwa-Jeong
(College of Pharmacy, Ewha Womans University)
- Published : 2003.04.01
Abstract
Unlike cationic liposome/DNA complexes, neutralliposomes containing plasmid DNA are stable in blood and does not selectively entrapped in the lung. The objective of this study was to construct neutral liposomes containing plasmid DNA with optimal encapsulation efficiency. Plasmid DNA (pGL2 clone 753,-6 kb) was encapsulated by the freeze/thawing method into liposomes composed of 1-palmitoyl-2-oleyl-sn-glycerol-3-phosphocholine(POPC), didodecyldimethylammonium bromide(DDAB), distaroylphosphatidyl-ethanolamine polyethylene glycol 2000(DSPE-PEG 2000) and DSPE-PEG 2000-maleimide. (omitted)
Keywords