한국식물병리학회:학술대회논문집 (Proceedings of the Korean Society of Plant Pathology Conference)
- 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
- /
- Pages.150.2-150
- /
- 2003
Characterization of Carnation mottle camovirus(CarMV) Isolated from Lilium spp. in Korea
- Park, J.H. (Dept. Biology, Yeungnam University) ;
- J.H. Sung (Dept. Biology, Yeungnam University) ;
- H.Y. Shin (Dept. Biology, Yeungnam University) ;
- M.U. Chang (Dept. Biology, Yeungnam University) ;
- S.N. Yoo (Dept. Plant Biotechnology, Catholic University)
- 발행 : 2003.10.01
초록
Carnation mottle carmovirus(CarMV) was isolated from Lilium spp. in Korea. This isolate, CarMV, was done bioassay, which plants were Dianthus caryophyllus, Gomphrena globosa, Chenopodium amaranticolor, Dianths chinensis. CarMV was propagated on the leaves of Chenopodium amaranticolor with the crude-sap inoculation method and purified by Mossops method(1976). We produced antiserum against CarMV and analyzed the antiserum specificity with ELISA, Gel diffusion method, and Rapid Immunofilter Paper Assay (RIPA). From these results of the assay, RIPA method was simple and rapid for CarMV detection. We have established successfully the CarMV detection system. CarMV coat protein gene was amplified by RT-PCR with specific primers and sequencing analysis was done.
키워드