Multiplex Reverse Transcription Polymerase Chain Reaction Assay for Simultaneous Detection of Five Cucurbit-infecting Viruses.

  • Lee, Su-Heon (Plant Pathology Division, National Institute of Agricultural Science and Technology) ;
  • Kim, Sang-Mok (Plant Pathology Division, National Institute of Agricultural Science and Technology) ;
  • Kim, Woo-Chang (JENO BIOTECH. Inc.) ;
  • Lee, Key-Woon (Department of Agricultural Biology, Kyungpook National University)
  • 발행 : 2003.10.01

초록

A single-step multiplex reverse transcription polymerase chain reaction (RT-PCR) assay was developed for the simultaneous detection of five cucurbit-infecting viruses: cucumber mosaic virus (CMV), watermelon mosaic virus 2 (WMV2), zucchini yellow mosaic virus (ZYMV), cucumber green mottle mosaic virus (CGMMV), and kyuri green mottle mosaic virus (KGMMV). The multiplex RT-PCR provides a simple and rapid method for detecting various viruses in cucurbit plants, which will help diagnose many cucurbit plants at a time.

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