Proceedings of the Korean Society of Developmental Biology Conference (한국발생생물학회:학술대회논문집)
- 2003.10a
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- Pages.100-100
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- 2003
Functional Cardiomyocytes Formation Derived from Mouse Embryonic Stem Cells
- Shin, Hyun-Ah (Maria Infertility Hospital Medical Institute/Maria Biotech) ;
- Lee, Keum-Sil (Maria Infertility Hospital Medical Institute/Maria Biotech) ;
- Cho, Hwang-Yoon (Maria Infertility Hospital Medical Institute/Maria Biotech) ;
- Park, Sae-Young (Maria Infertility Hospital Medical Institute/Maria Biotech) ;
- Kim, Eun-Young (Maria Infertility Hospital Medical Institute/Maria Biotech) ;
- Lee, Young-Jae (Maria Infertility Hospital Medical Institute/Maria Biotech) ;
- Park, Se-Pill (Maria Infertility Hospital Medical Institute/Maria Biotech) ;
- Lim, Jin-Ho (Maria Infertility Hospital)
- Published : 2003.10.01
Abstract
Pluripotent embryonic stem (ES) cells differentiate spontaneously into beating cardiomyocytes via embryo-like aggregates. We describe the use of mouse embryonic stem (mES03) cells as a reproducible differentiation system for cardiomyocyte. To induce cardiomyocytic differentiation, mES03 cells were dissociated and allowed to aggregate (EB formation) at the presence of 0 75% dimethyl sulfoxide (DMSO) for 4 days and then another 4 days without DMSO (4+/4-). Thus treated EBs were plated onto gelatin-coated dish for differentiation. Spontaneously contracting colonies which appeared in approximately 4-5 days upon differentiation. Expression of cardiac-specific genes were determined by RT-PCR. Rebust expression of myosin light chain (MLC-2V), cardiac myosin heavy chain