참당귀 뿌리 배양에 있어서 root segmentation과 식물생장조절제가 뿌리 생장과 decursinol angelate 생산에 미치는 영향

Decursinol angelate, a new anticancer agent, was produced by root cultures of Angelica gigas Nakai. In addition, difference of specific yields between primary and secondary root was investigated. It was found that specific yield of secondary root was much higher than that of primary root at various conditions, so that it was thought that the formation and growth of secondary root were feasible. From this point of view, effects of root segmentation and plant growth regulators (NAA, IBA) on root morphology and decursinol angelate production were examined. Root segmentation increased secondary root mass and product formation. On the other hand, addition of NAA or IBA at various concentrations promoted secondary root formation and production of decursinol angelate significantly. Five-fold increase of production was obtained at 4 mg/L of IBA compared to control without NAA and IBA.

접종 초기 root segmentation 은 뿌리의 생장 속도를 감소시키고 그 생산성을 저하시키기도 하지만 F/D ratio를 감소시켜 뿌리가 자랄 수 있는 배지 내 공간을 제공하기 때문에 고농도 배양에 유리할 뿐 아니라, 생산성이 높은 secondary root의 비율을 증가시키기 때문에 decursinol angelate 의 생산량을 중대시킨다. 참당귀 뿌리배양을 위한 plant growth regulator의 최적 조건으로 secondary root의 생장성 측면이나 decursinol angelate 의 생산성 측면에서 4 mg/L 이상의 IBA 첨가가 요구됨을 확인하였다.