Bubble-column Photobioreactor에서의 Astaxanthin 유도배양의 연구

This study investigated a lab-scale inducing method for efficient astaxanthin accumulation. As a model system. Haematococcus pluvialis was cultivated in 2 liter bubble-column photobioreactors. The astaxanthin - inducing results using high light irradiation were compared with that of the control experiment under standard irradiation (40 ${\mu}E/m^2/s$). After the late linear growth phase (> 20 days). high light energy (230 ${\mu}E/m^2/s$) was supplied to the culture broth for astaxanthin induction. As a result. the dr γ cell weight and the astaxanthin productivity were increased up to 68% and 215%, respectively. higher than those of the control experiment. This result indicates that bubble-column type photobioreactor is a good candidate for mass cultivation of H. pluvialis and high light irradiation is an efficient induction method for astaxanthin accumulation in lab-scale bubble-column photobioreactors.

본 연구에서는 자체 제작한 2L 규모의 bubble-column photobioreactor에서 H. pluvialis의 배양을 시도하였고 astaxanthin의 축적량 증가를 유도하기 위하여 배양시작 20일 후에 light stress를 주었다. 이 방법을 통하여 대조구에 비하여 68%의 건조균체중량 증가와 215%의 astaxanthin 축적량 증가를 유도할 수가 있었고, bubble-column photobioreactor를 이용한 유도배양이 H. pluvialis의 배양에 적합함을 알 수가 있었다.