Proceedings of the PSK Conference (대한약학회:학술대회논문집)
- 2002.10a
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- Pages.426.3-427
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- 2002
Enhancement of Emulsion-mediated Gene Expression by Using Chitosan as a Pre-Condensing Agent
- Chun, Soo-Kyung (College of pharmacy, Seoul national University) ;
- Choi, Sung-Hee (College of pharmacy, Seoul national University) ;
- Kim, Adele (College of pharmacy, Seoul national University) ;
- Ahn, Woong-Shick (College of Medicine, the Catholic University of Kore) ;
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Kim, Chong-Kook
(College of pharmacy, Seoul national University)
- Published : 2002.10.01
Abstract
The aim of this study was to enhance the transfection efficiency of emulsion-mediated gene expression by using chitosan, Conventional DNA/emulsion complexes and precondensed DNA/emulsion complexes were prepared by adding either naked or precondensed plasmids to cationic emulsion. The zeta potential. TEM, and size of transfection complexes were measured. In vitro transfection efficiency for boty complexes was also studied by several methods: flow cytometer, expression analysis by confocal microscope, RT-PCR, and in addition. (omitted)
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