Proceedings of the PSK Conference (대한약학회:학술대회논문집)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Simultaneous Determination of Curcumin and Glycyrrhizin Contents by High-performance Liquid Chromatography in Two Different Oriental Herbal Preparations of Kamijadowhan

  • Kim, Eun-Young (Toxicology Lab., Korea Institute of Science and technology) ;
  • Choi, Seung-Hun (Department of Pathology, college of Oriental Medicine, Kyung Hee University) ;
  • Kwon, Oh-Seung (Toxicology Lab., Korea Institute of Science and technology)
  • Published : 2002.10.01
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Abstract

A high-performance liquid chromatographic method was developed to determine the quantities of curcumin and glycyrrhizin in two different oriental herbal preparations of Kamijadowhan (KMD. NKMD). Two compounds were separated in less than 10 min with a Nova-Pak $C_{18}$ column ($3.9{\times}150$ mm ($5{\mu}$ particle size) by linear gradient elution using 0.03% (v/v) phosphoric acid-acetonitrile (60:40. v/v% at 0 min; 40:60 v/v% at 6 mini as the mobile phase at a flow-rate of 0.8 ml $min^{-1}$. (omitted)

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