Proceedings of the PSK Conference (대한약학회:학술대회논문집)
- 2002.10a
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- Pages.332.2-333
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- 2002
Three alkaline proteases of Bacillus spp. JB411.
- Lee, Oh-Hyoung (Department of Biology, Mokpo National University) ;
- Seong, Chi-Nam (Department of Biology, Sunchon National University) ;
- Park, Gi-Duk (Department of Pharmacy, College of Pharmacy, Chosun University) ;
- Kim, An-Na (Department of Pharmacy, College of Pharmacy, Chosun University) ;
- Han, Ji-Man (Department of Pharmacy, College of Pharmacy, Chosun University) ;
- Lee, Hyo-Jung (Department of Pharmacy, College of Pharmacy, Chosun University) ;
- Kim, Sul-Hee (Department of Pharmacy, College of Pharmacy, Chosun University) ;
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Yoo, Jin-Cheol
(Department of Pharmacy, College of Pharmacy, Chosun University)
- Published : 2002.10.01
Abstract
Three alkaline proteases. designated JB-1. JB-2, and JB-3, are extracellular enzymes produced by Bacillus spp. JB411 which was isolated korean soil. They were separated by DEAE-sepharose CL-6B gel. and further purified using ammonium sulfate precipitation. ultra membrane filtration. and Ultrogel AcA gel filtration. The optimun pH values of proteases IB-1. JB-2. and JB-3. were shown to be 9.5. 9.5 and 7.5. respectively. All three proteases were stable in the pH range of 5-11. (omitted)
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