Revers phase HPLC Separation of D-Amygdalin and Neoamygdalin and Optimum Conditions for Inhibition of Racemization of Amygdalin

  • Hwang, Eun-Young (Department of Oriental Pharmaceutical Sciences, Kyung Hee University) ;
  • Kim, Dong-Min (College of Pharmacy, Chungbuk National University) ;
  • Koh, Jung-A (College of Pharmacy, Chungbuk National University) ;
  • Lee, Sang-Soo (College of Pharmacy, Chungbuk National University) ;
  • Lee, Je-Hyun (College of Pharmacy, Chungbuk National University) ;
  • Lee, Yong-Moon (College of Pharmacy, Chungbuk National University) ;
  • Hong, Seon-Pyo (College of Pharmacy, Chungbuk National University)
  • Published : 2002.10.01

Abstract

In boiling aqueous solution, D-amygdalin usually begins to convert into neoamygdalin in 3 min and more than 30% of the initial D-amygdalin is found as neoamygdalin after 30 min. In this report, we establish methods for simple HPLC analysis and the inhibition of D-amygdalin conversion. D-Amygdalin and its conversion product, neoamygdalin, were clearly separated on reverse-phase column chromatography by an optimized eluent of 10mM sodium phosphate buffer (pH 3.8) containing 6% acetonitrile. (omitted)

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