Effect of Mammalian Spermatozoa on In Vitro Maturation of Porcine Germinal Vesicle Oocyte in Chemically Defined Medium

  • Published : 2002.06.01

Abstract

Oocytes maturation, characterized by germinal vesicle (GV) breakdown, formation of the first meiotic spindle, expulsion of the first polar body and arrest in metaphase of second meiotic division (MII), occurs in preovulatory follicles in response to the surge of gonadotropin and leads to an ovulated oocyte in vivo. However, meiotic resumption in vitro occurs spontaneously following removal of cumulus-oocytes complexes (COCs) from the follicle. (omitted)

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