Effects of Antioxidants on the Antioxidant and Apoptosis Genes Expression of IVM/IVF Hanwoo Embryos

  • Jang, H.Y. (College of Animal Resource Science, Kangwon National University) ;
  • Parkm C.K. (College of Animal Resource Science, Kangwon National University) ;
  • Cheong, H.T. (College of Animal Resource Science, Kangwon National University) ;
  • Kim, C.I. (College of Animal Resource Science, Kangwon National University) ;
  • Yim, S.V. (Department of Pharmacology, Kangwon National University) ;
  • Kim, S.S. (College of Medicine, Kangwon National University) ;
  • Park, D.H. (College of Animal Resource Science, Kangwon National University) ;
  • Yang, B.K. (College of Animal Resource Science, Kangwon National University)
  • Published : 2002.06.01

Abstract

To investigate the effects of two antioxidants, aesculeitin and taurine, expression of apoptosis related genes and antioxidant enzyme gene in preimplantation Hanwoo embryos was determined by modified semi-quantitative single cell RT-PCR. Hanwoo embryos derived from in vitro maturation /in vitro fertilization were cultured in 5% CO₂ and 5% O₂ in CR₁aa medium at 37℃. Aesculeitin and taurine were added to medium at concentration of l㎍/㎖ and 2.5mM, respectively. (omitted)

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