Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)
- 2002.06a
- /
- Pages.52-52
- /
- 2002
Studies on In Vitro Developmental Rate of Activated Bovine Oocytes by Intracytoplasmic Sperm Injection with Frozen-Thawed Epididymal Spermatozoa
- Lee, Dongsoo (College of Vet. Med., Chungnam National University) ;
- Kim, Sangkeun (College of Vet. Med., Chungnam National University)
- Published : 2002.06.01
Abstract
The objective of this study was to determine the developmental competence of in vitro matured bovine oocytes after intracytoplasmic sperm injection(ICSI) with frozen-thawed epididymal spermatozoa. The ovaries were obtained from slaughtered Korean native cows. Oocytes matured in vitro for 24 hrs were fertilized by ICSI with frozen-thawed epididymal spermatozoa. After ICSI, one group of oocytes was activated with 7% ethanol for 5 min, and second group was not activated. The follicular oocytes were cultured in TCM-199 medium containing hormones and 10% FCS for 24-30 hrs in a incubator with 5% CO₂ in air at 38.5℃. (omitted)