한국가금학회:학술대회논문집 (Proceedings of the Korea Society of Poultry Science Conference)

한국가금학회 (The Korean Society of Poultry Science)
권호 표지

The Production of Germline Chimeras by Transfer of Gonadal Primordial Germ Cells Separated with Magnetic Cell Sorter System in Quail

  • Shin, Sang-Su (School of Agricultural Biotechnology, Seoul National University) ;
  • Park, Tae-Sub (School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Mi-Ah (School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Jin-Nam (School of Agricultural Biotechnology, Seoul National University) ;
  • Lee, Kyung-Joo (School of Agricultural Biotechnology, Seoul National University) ;
  • Park, Hyun-Jeong (School of Agricultural Biotechnology, Seoul National University) ;
  • Han, Jae-Yong (School of Agricultural Biotechnology, Seoul National University)
  • 발행 : 2002.11.01
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초록

Collection of large number of gonadal Primordial germ cells(gPGCs) is a prerequisite factor for improving germline transmission efficacy in the aves, In this study a magnetic-activated cell sorter(MACS) was applied for improving retrieval efficacy of quail gPGCs and the migration capacity of MACS-separated gPGCs was further examined after being transplanted to recipient embryos. We also induced germline chimeras by transfer of MACS-separated quail gPGCs at the efficiency of 17.4% on average.

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