한국가금학회:학술대회논문집 (Proceedings of the Korea Society of Poultry Science Conference)
- 한국가금학회 2002년도 가을 학술발표논문집
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- Pages.96-97
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- 2002
Derivation of primordial germ cells from chicken blastodermal cells by BMP-2 and BMP-4 signaling
- Kim, Duk-Kyung (School of Agricultural Biotechnology, Seoul National University) ;
- Song, Ki-Duk (School of Agricultural Biotechnology, Seoul National University) ;
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Lee, Young-Mok
(School of Agricultural Biotechnology, Seoul National University)
;
- Seo, Sam-Youl (School of Agricultural Biotechnology, Seoul National University) ;
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Han, Jae-Yong
(School of Agricultural Biotechnology, Seoul National University)
- 발행 : 2002.11.01
초록
Primordial germ cells (PGCs) are the progenitors of the sperms or eggs of adult. Evidence suggests that the specification of primordial germ cells (PGCs) in the mammalian embryo does not depend on maternal determinants. Recent previous studies in the mouse has shown that several bone morphogenetic proteins (BMPs) are required for the formation of PGCs. However, there is no study about the effect of BMPs on avian PGCs. Here, we studied the effects of recombinant human BMP-2 (rhBMP-2) and recombinant human BMP-4 (rhBMP-4) on chicken blastodermal cells in culture. As a results, the addition of rhBMP-2 and rhBMP-4 increased the number of SSEA-1 positive cells in dose-dependent manner. However, there is no synergic effect by using both rhBMP-2 and rhBMP-4.