Proceedings of the Korean Society of Toxicology Conference (한국독성학회:학술대회논문집)
- 2001.10a
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- Pages.189-189
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- 2001
SIMULTANEOUS EXPRESSION OF HUMAN CYTOCHROME P450 3A5 AND NADPH-CYTOCHROME P450 REDUCTASE IN CHINESE HAMSTER OVARY CELL USING INTERNAL RIBOSOME ENTRY SITE
- Kang, Hyuck-Joon (College of Medicine and Institute of Biomedical Sciences, Hanyang University) ;
- Kang, Jin-Sun (College of Medicine and Institute of Biomedical Sciences, Hanyang University) ;
- Dong, Mi-Sook (Graduate School of Biotechnology, Korea University) ;
- Park, Chang-Hwan (College of Medicine and Institute of Biomedical Sciences, Hanyang University)
- Published : 2001.10.01
Abstract
For a continuous expression of human cytochrome p450 3A5 (CYP3A5) and NADPH-cytochrome P450 reductase (CYPR) proteins, bicistronic construct (CYP3A5BC-LNCX2) was made using internal ribosome entry site (IRES). As for mammalian cell expression, we used pLNCX2 retroviral vector; and using calcium phosphate, plasmid transfer was achieved in 293GPG cell and transduced in Chinese hamster ovary (CHO) cell.(omitted)
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