Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)
- 2001.03a
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- Pages.42-42
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- 2001
Production of Embryos by Intracytoplasmic Sperm Injection (ICSI) in Pig
- Cho, Hwang-Yun (Division of Applied Life Science, Gyeongsang National University) ;
- Cho, Seong-Keun (Division of Applied Life Science, Gyeongsang National University) ;
- Park, Mi-Ryung (Division of Applied Life Science, Gyeongsang National University) ;
- Kim, Jung-Ho (Division of Applied Life Science, Gyeongsang National University) ;
- Kim, Jin-Hoi (Division of Applied Life Science, Gyeongsang National University)
- Published : 2001.03.01
Abstract
The objective of this study was to assess the development of porcine follicular oocytes fertilized by ICSI. Cumulus-oocyte-complexes (COCs) were collected by aspiration from follicles of 2-7 mm in diameter from a local slaughterhouse ovaries. Oocytes matured for 40-44 h were centrifuged at 12,000g for 6 min and then injected with sperm prepared by swim-up procedure in the presence or absence of 5 mM dithiothreitol (DTT). Injected oocytes were cultured in NCSU 23 medium during 6 to 8 days. IVF controls were compared to those of resulting embryos. The results obtained were as. follow: 1, The rates of cleavage and development rates into blastocyst by ICSI were not significantly (P<0.05) different between with (53.0% and 19.7%) or without (48.3% and 23.8%) centrifugation, respectively. 2. The cleavage and developmental rates to blastocyst after ICSI with or without 5mM DTT treated-sperm were not significantly (P<0.05) different (60.4% vs 16.4% and 48.5% vs 22.2%, respectively). 3. The cleavage and the developmental rates to blastocyst were not significantly (P<0.05) different between the zygotes obtained by IVF (51.8% vs. 22.4%) and ICSI (51.4% vs. 21.6%). 4. The number of blastomere in blastocyst stages after IVF or ICSI was not significantly different (46.7