Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)
- 2001.03a
- /
- Pages.20-20
- /
- 2001
Aberrant Distributions of ICM Cells in Bovine Blastocysts Produced by Somatic Cell Nuclear Transfer
- D. B. Koo (Animal Developmental Biotechnology Lab. Korea Research Institute of Bioscience & Biotechnology) ;
- Y. K. Kang (Animal Developmental Biotechnology Lab. Korea Research Institute of Bioscience & Biotechnology) ;
- Park, Y. H. (Animal Developmental Biotechnology Lab. Korea Research Institute of Bioscience & Biotechnology) ;
- Park, J. S. (Animal Developmental Biotechnology Lab. Korea Research Institute of Bioscience & Biotechnology) ;
- Kim, H. N. (Animal Developmental Biotechnology Lab. Korea Research Institute of Bioscience & Biotechnology) ;
- D. S. Son (National Livestock Research Institute) ;
- Y. M. Han (Animal Developmental Biotechnology Lab. Korea Research Institute of Bioscience & Biotechnology) ;
- Lee, K. K. (Animal Developmental Biotechnology Lab. Korea Research Institute of Bioscience & Biotechnology)
- Published : 2001.03.01
Abstract
It has been reported that cloning cattle is inefficient. One of the problems was placental abnormality, finally resulting in fetal mortality after transfer of nuclear transfer (NT) bovine embryos. This study was focused on the allocations of embryonic cells to the inner cell mass (ICM) or to the trophectoderm(TE) in NT bovine blastocysts. Somatic cells were derived from a Day 45 fetus of gestation, individually transferred into enucleated oocytes and developed to the blastocyst stage in vitro. Differential staining was used to assess the qualify of blastocysts derived from NT, IVF and in vivo. Development rate of NT embryos to blastocysts (25.0%, 41/164) was similar to that of IVF embryos (28.7%, 49/171). The total cell number of NT blastocysts (101.3