한국생물공학회:학술대회논문집

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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재조합 효모를 이용한 endoinulinase의 생산 특성

  • 한지혜 (전북대학교 화학공학부) ;
  • 이은미 (전북대학교 화학공학부) ;
  • 윤영미 (전북대학교 화학공학부) ;
  • 이현철 (한려대학교 신소재공학과) ;
  • 정봉우 (전북대학교 화학공학부) ;
  • 채건상 (전북대학교 생물과학부)
  • Published : 2000.04.08
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Abstract

The INU2 gene encoding an endoinulinase of Aspergillus ficuum was expressed by the Kluyveromyces marxianus INU1 promoter in a SUC2-deleted Saccharomyces cerevisiae to produce the endoinulinase free of an exoinulinase and an extracellular invertase in the culture medium. When inulin was included in the medium, a recombinant yeast strain produced the sufficient amount of the enzyme to make a halo around its colony. An expression of endoinulinase was dependent on the culture temperature and shaking. The highest expression of endoinulinase was observed at $30^{\circ}C$, and 150rpm.

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