Actinoplanes teichomyceticus의 변이주에 의한 Teicoplanin 발효생산

  • 김성건 (영동대학교 생명공학부 유전공학과) ;
  • 안현진 (영동대학교 생명공학부 유전공학과) ;
  • 김재영 (영동대학교 생명공학부 유전공학과) ;
  • 노용택 (영동대학교 생명공학부 유전공학과)
  • 발행 : 2000.04.08

초록

Actinoplanes teichomyceticus ATCC 31121 was mutated with UV to obtain a superior mutant strain with increasing level of teicoplanin production. In this investigation lethal curve was obtained and the optimal condition to induce mutagenesis was determined and to isolate the desirable mutant strain. It was also confirmed that teicoplanin activities by agar diffusion method to be compared to the mother strain. One mutant strain, T991014-1 that had the highest teicoplanin productivity was finally selected for further investigation including fermentation pattern. The mutant was characterized by the various tests such as amylase activity, protease activity, antibiotic resistance, autotoxicity, and productivity.

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