한국작물학회:학술대회논문집 (Proceedings of the Korean Society of Crop Science Conference)
- 한국작물학회 1999년도 춘계 학술대회지
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- Pages.162-163
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- 1999
Plant genome analysis using flow cytometry
- Lee Jai-Heon (Dong-A University, Faculty of Natural Resources and Life Science) ;
- Kim Kee-Young (Dong-A University, Faculty of Natural Resources and Life Science) ;
- Chung Dae-Soo (Dong-A University, Faculty of Natural Resources and Life Science) ;
- Chung Won Bok (Dong-A University, Faculty of Natural Resources and Life Science) ;
- Kwon Oh-Chang (Dong-A University, Faculty of Natural Resources and Life Science)
- 발행 : 1999.05.01
초록
The goal of this research was (1) to describe the conditions and parameters required for the cell cycle synchronization and the accumulation of large number of metaphase cells in maize and other cereal root tips, (2) to isolate intact metaphase chromosomes from root tips suitable for characterization by flow cytometry, and (3) to construct chromosome-specific libraries from maize. Plant metaphase chromosomes have been successfully synchronized and isolated from many cereal root-tips. DNA synthesis inhibitor (hydroxyurea) was used to synchronize cell cycle, follwed by treatement with trifluralin to accumulate metaphase chromosomes. Maize flow karyotypes show substantial variation among inbred lines. thish variation should be sueful in isolating individual chromosome types. In addition, flow cytometry is a useful method to measure DNA content of individual chromosomes in a genotyps, and to detect chromosomal variations. Individual chromosome peaks have been sorted from the maize hybrid B73/Mol7. Libraries were generated form the DOP-PCR amplification product from each peak. To date, we have analyzed clones from a library constructed from the maize chromosome 1 peak. Hybridization of labeled genomic DNA to clone inserts indicated that
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