Proceedings of the Botanical Society of Korea Conference (한국식물학회:학술대회논문집)
- 1999.04a
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- Pages.18-18
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- 1999
Characterization of a Low Molecular Weight Heat-Shock Protein cDNA Clone from Nicotiana tabacum
- Park, Soo-Min (Institute for Molecular Biology and Genetics, Seoul National University) ;
- Joe, Myung-Kuk (Institute for Molecular Biology and Genetics, Seoul National University) ;
- Hong, Choo-Bong (Institute for Molecular Biology and Genetics, Seoul National University)
- Published : 1999.04.01
Abstract
We characterized a cDNA clone for a low molecular weight heat-shock protein (LMW HSP) from tobacco named TLHS-l. Nucleotide sequence determination of TLHS-1 identified an open reading frame for 159 amino acids. To the upstream of the open reading frame, a sequence of 124 nucleotides was determined. To the 3' downstream of the open reading frame, 212 nucleotides were identified which carried poly(A)-tail. Comparison of the open reading frame and hydropathy plot of TLHS-1 with the previously reported class I LMW HSPs showed high identity which classified TLHS-1 as a class I LMW HSP cDNA clone. We proposed that there are six consensus regions in class I LMW HSPs. RNA blot hybridization for TLHS-1 showed a typical expression pattern of heat-shock-inducible gene from three common tobacco cultivars. The open reading frame of TLHS-1 was overexpressed in Escherichia coli. TLHS-1 protein confers thermal protection of other proteins in vitro and in vivo. Thermal induced aggregation of citrate synthase was reduced by purified TLHS-1 protein, and thermal death rate at