대한의용생체공학회:학술대회논문집 (Proceedings of the KOSOMBE Conference)

대한의용생체공학회 (The Korean Society of Medical and Biological Engineering)
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Evaluation of the platelet adhesiveness using a peptide-immobilized surface

  • Kim, J.H. (Institute of Biomedical Engineering, College of Medicine, Seoul National University) ;
  • Kim, H.J. (Institute of Biomedical Engineering, College of Medicine, Seoul National University) ;
  • Kim, J.W. (Biomedlab. Co.) ;
  • Min, B.G. (Institute of Biomedical Engineering, College of Medicine, Seoul National University) ;
  • Choe, T.B. (Department of Microbiological Engineering, College of Engineering, Kon-kuk University)
  • 발행 : 1997.05.23
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초록

The adhesion of stimulated and unstimulated platelet to fibrinogen requires the receptor binding site of GPIIb/IIIa. These recognition sites are existed in the Au chain(RGDS at positions 572-575 and RGDF at 95-98) and the carboxyterminal $\gamma$ chain (HHLGGAKQAGDV at 400-411) of fibrinogen. The unstimulated platelet does not adhered on the fragment E-coated surface containing RGDF sequence. In this study, we developed RGDF-immobilized surface to detect the functional state of platelet. RGDF-immobilized surface was prepared on the glass using photolithographic technology. Platelet adhesion to petide(RGDF)-immobilized surface was observed by the fluorescence microscope using mepacrine.

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