Proceedings of the KOSOMBE Conference (대한의용생체공학회:학술대회논문집)

The Korean Society of Medical and Biological Engineering (대한의용생체공학회)
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Analysis and Measurement of the Spectrum of Whole Blood

전혈의 SPECTRUM 측정과 분석

  • Kim, Y.J. (Biomedical Engineering Research Center, Samsung Biomedical Research Institute) ;
  • Kim, H.S. (Biomedical Engineering Research Center, Samsung Biomedical Research Institute) ;
  • Kim, J.W. (Department of Clinical Pathology, Samsung Medical Center) ;
  • Yoon, K.W. (Biomedical Engineering Research Center, Samsung Biomedical Research Institute) ;
  • Kim, W.K. (Biomedical Engineering Research Center, Samsung Biomedical Research Institute)
  • 김연주 (삼성생명과학연구소 임상의공학센타) ;
  • 김흥식 (삼성생명과학연구소 임상의공학센타) ;
  • 김종원 (삼성의료원 임상병리과) ;
  • 윤길원 (삼성생명과학연구소 임상의공학센타) ;
  • 김원기 (삼성생명과학연구소 임상의공학센타)
  • Published : 1996.05.17
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Abstract

The spectra of whole blood EDTA samples from two people were generated using a CARY 5E (UV-VIS-NIR) spectrophotometer from 400 to 1000nm which contain visible and NIR region. Only the data between 400 and 800nm were used to analyze the components of blood. Using the same spectrophotometer, the spectra of Water, normal saline, plasma were generated These spectra were subtracted from each blood sample, and then the first derivative of each of the subtracted data was taken to minimize baseline variations and indicated the wavelength-shift of peak and valley. Normalization and division between two blood samples were used to correlate the quantity ratio of specific components with feature of spectra. Samples were controlled at $30^{\circ}C,\;37^{\circ}C$, ambient temperature.

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