Proceedings of the Korean Biophysical Society Conference (한국생물물리학회:학술대회논문집)

The Korean Biophysical Society (한국생물물리학회)
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Cloning, Expression, and Characterization of Superoxide dismutase from Aquifex Pyophilus, a Hyperthermophilic Bacteria

  • Rhim, Jae-Hwan (Structural Biology Center, KIST) ;
  • Yesun Han (Structural Biology Center, KIS) ;
  • Kim, Sung-Hou (Department of Chemistry and Lawrence Berkeley National Laboratory, University of Califoni) ;
  • Yunje Cho (Structural Biology Center, KIST)
  • Published : 1996.07.01
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Abstract

A suproxide dismutase gene of Aquifex pyroprolus, a novel marine hypenhermophilic bacterium, was cloned, expressed, and characterized. The SOD of A pyrophilus (ApSOD) is an iron-containing homo-oligomeric protein with a monomeric molecular weight of 24.2 kDa. the amino acid sequence is similar to those of known Mn- and Fe-SODs from thermophilic archaea, and metal binding residues in all SOD sequences from different species are also conserved in A. pyrophilus SOD. (omitted)

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