• Journal of Dairy Science and Biotechnology
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• v.34 no.4
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• pp.239-244
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• 2016

The roots of Cichorium intybus L. (chicory) have many nutritional qualities and also have been used for health promotion as traditional medicine. The antimicrobial activity of Cichorium intybus L.'s (chicory) ethanol extract on Bacillus cereus ATCC 10876 were investigated using spot on lawn method in this study. Ethanol extract exhibited antimicrobial activities against Bacillus cereus ATCC 10876 with significance. Also, the results produced the larger zones of inhibition against Bacillus cereus ATCC 10876 tested, with increasing concentration of Cichorium intybus L.'s (chicory) ethanol extracts,. Hence, Cichorium intybus L. (chicory) could be served for keeping the safety of various dairy products as natural antimicrobial agents.

• Journal of Sasang Constitutional Medicine
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• v.16 no.3
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• pp.85-95
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• 2004

1. Objectives Although the incidence of allergic rhinitis is increasing continually, Sasang Constitutional Medicine is showing favorable treatment outcomes. This study was aimed to test the antimicrobial effects of Bojungikgi-tang, Gwakhyangjeonggi-san, and Gunggwihyangso-san which are being applied to allergic rhinitis of Soeumin. 2. Methods Klebsiella pneumoniae 289 was extracted from allergic rhinitis patients. Bojungikgi-tang, Gwakhyangjeonggi-san, and Gunggwihyangso-san were boiled down and concentrated, and the substances obtained were tested for the effects on stability to temperature and pH and on antimicrobial activity 3. Results When measured the level of antimicrobial activity, the inhibition zones of Bojungikgi-tang, Gwakhyangjeonggi-san, and Gunggwihyangso-san were found 11.42, 11.07, and 10.63mm, respectively. The levels of MIC were $15{\mu}{\ell}$, $20{\mu}{\ell}$, and $10{\mu}{\ell}$ in that order. The antimicrobial activities were increased 13.88% in Bojungikgi-tang, 16.63% in Gwakhyangjeonggi-san, and 10.72% in Gunggwihyangso-san. There was no influence of pH to antimicrobial activity of all three substances. 4. Conclusions Bojungikgi-tang, Gwakhyangjeonggi-san, and Gunggwihyangso-san had a high level of antimicrobial activity to Klebsiella pneumoniae 289 even at a low concentration.

• Korean journal of food and cookery science
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• v.25 no.4
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• pp.496-505
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• 2009

The influence of roasting time on antibacterial and antioxidative effects of methanol and water coffee extracts was investigated. Extract yield differed with roasting time. The maximum yield of methanol extract was 20.02% and 24.00% at respective roasting times of 12 and 20 min. The maximum yield of water extracts was 2.70% and 18.58% at 5 and 25 min roasting time, respectively. Antibacterial effects of each extract were determined by the classical minimal inhibitory concentration (MIC) paper disc diffusion method. Methanol extracts of different coffee samples inhibited growth of various strains except Escherichia coli. Extracts obtained following roasting times of 12, 14, 16, 20, and 25 min in particular displayed the most potent activity against Staphylococcus aureus. Among these extracts, that obtained from 12 min roasted coffee samples produced a MIC of $16.125{\mu}g$/mL against S. aureus. Water extracts applied at $1,000{\mu}g$/mL were growth inhibitory except against Salmonella choleraesuis and Prevotella intermedia. However, growth inhibition by water extracts was weak, with inhibitory zones of only 6-8 mm diameter produced. Determinations of free radical elimination for the different coffee extracts using 1,1-diphenyl-2-picrylhydrazyl were compared with ascorbic acid and butylated hydroxytoluene positive controls. Methanol and water extracts of different coffee samples ($100{\mu}g$/mL) showed $67.1{\sim}92.3%$ and $66.4{\sim}93.3%$ radical scavenging activity, respectively. However, longer roasting time (especially >20 min) tended to somewhat lower free radical elimination using both extracts. Total phenol in different coffee samples measured by the Folin-Denis method revealed the highest level of phenol contents with non-roasted coffee, whereas phenol content differed with different roasting time, ranging from $87.{\sim}126.5\;mg/g$ in methanol extracts. In water extracts, the phenol content was maximum at 8 min roasting time, whereas in other samples the content was varied from $95.0{\sim}199.1\;mg/g$.

• The Journal of Natural Sciences
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• v.11 no.1
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• pp.159-164
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• 1999

The study was investigated to the effect of antimicrobial finishing of towel treated by organic antimicrobial agent. It was measured for the optimal condition such as treated time, treated concentration and temperature. After amtimicrobial treatment, mechanical and fastness properties, anti-laundering property were measured.The antimicrobial activity of samples was analyzed quantitatively by masureing the number of colonies of Staphylococcus aureus using the shake flask method. Towel samples treated with the optimal condition such as treated time of 10 minute, and treated temperature of $30^{\circ}C$, concentration of organic antimicrobial agent of 3.5% were shown a high reduction rate in the number of colonies grown and clear zones of inhibition.The effect of reduction rate for laundering until the number of 20 times was shown high reduction rate of over 80%. And the mechanical properties of samples treated with organic antimicrobial agent were not changed approximately.

• Journal of the East Asian Society of Dietary Life
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• v.22 no.5
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• pp.692-700
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• 2012

The antibacterial activity of the ethanol extract of Cornus officinalis against some bacteria related to foodborne illness and food spoilage was investigated. In the case of the disc diffusion assay, clear zones due to the inhibition of proliferation by Cornus officinalis extract were 8.5~18.3 mm at $4,000{\mu}g/disc$. The inhibitory effect of Cornus officinalis was as follows: Escherichia. coli ${\geq}$ Listeria monocytogenes ${\geq}$ E. coli O157:H7 > Bacillus cereus > Staphylococcus aureus > Pseudomonas aeruginosa > Salmonella typhimurium. In addition, the MIC of Cornus officinalis extract toward B. cereus was the lowest at 1,000 ppm. The extract inhibited the growth of E. coli, L. monocytogenes, E. coli O157:H7, and Bacillus cereus throughout the growth stage. However, the growth of P. aeruginosa, S. aureus, and S. typhimurium was only inhibited by the extract during the lag phase. The constituents of all cells tested were released, especially E. coli and E. coli O157:H7. Observation of the cells using SEM demonstrated a morphological change and disruption of cells in response to treatment with Cornus officinalis extract. Based on these findings, the ethanol extracts of Cornus officinalis showed strong antimicrobial activity against all tested bacteria, indicating that Cornus officinalis can be a useful natural antimicrobial reagent.

• Analytical Science and Technology
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• v.30 no.3
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• pp.113-121
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• 2017

Now a day's rise of new antibiotic resistant bacterial strains is a global threat. Ethnic people of India have been employing Aphanamixis polystachya (Wall.) R. Parker wood extract in healing cancerous wounds. The aim of this study was to evaluate the antimicrobial activity and to identify the medicinally potent chemicals in the essential oil extract of A. polystachya. The antibacterial properties of various organic extracts were evaluated against a range of bacteria (gram-positive and gram-negative bacteria) based on the disc diffusion method and GC-MS based analysis for finding active oil extract components. All extracts of A. polystachya leaves showed potential antibacterial activity, notably ethyl acetate, while petroleum ether extracts revealed highly sensitive activity against all tested bacteria (zones of inhibition ranging from 8.83 to 11.23 mm). In addition, the petroleum ether extract had the lowest MIC value (32 to $256{\mu}g/mL$) against E. coli, S. lutea, X. campestris, and B. subtilis bacteria. The major compounds detected in oil [${\beta}$-elemene (16.04 %), ${\beta}$-eudesmol (12.78 %), ${\beta}$-caryophyllene (19.37 %), ${\beta}$-selinene (11.32 %), elemol (5.76 %), and ${\alpha}$-humulene (5.68 %)] are expected to be responsible for the potent antimicrobial activity. The results of this study offer valuable insights into the potent role of A. polystachya essential oil extract in pharmaceutical and antibiotic research.

• Korean journal of applied entomology
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• v.9 no.2
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• pp.75-80
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• 1970

1. Retention of fungicides and efect of the mixing of spreaders and insecticides with fungicides in control of apple bitter rot were evaluated by 'the assay of inhibition zones with apple exocarp disks:' 2. The effectiveness of chemicals was reduced gradually as the time after treatment increased. Of all the chemicals tested, Difolatan retained approximately 60 percent of the original activity even after IS days. Difolatan had the highest followed by Tuzet, Phaltan, Bordeaux mixture, and Delan, in that order. 3. The fungicidal acivity of Tuzet decreased with increasing application of simulated rain. The wash-offf of Tuzet was reduced by adding spreaders. Dry skim milk and soybean extract were better than commercial chemicals such as Lino No. 1,2 and Tween 20. 4. The mixing of insecticides such as EPN, Folithion, Parathion and Lebaycid with Phaltan resulted in no significant differences in fungicidal effect even after 12 days of storage at room temperature.

• Journal of Pharmacopuncture
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• v.19 no.3
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• pp.246-252
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• 2016

Objectives: The purpose of this study was to obtain a natural antibiotic from Phenol-rich compounds; for the dressing and the treatment of chronic wounds. Methods: The Phenol-rich compound sweet gel was prepared by blending four natural herbal extracts, Acacia catechu (L.F.), Momia (Shilajit), Castanea sativa, and Ephedra sinica stapf, with combination of a sweet gel medium, including honey, maple saps, Phoenix dactylifera L. (date), pomegranate extract and Azadirachta indica gum as a stabilizer. The combinations were screened by using a well-diffusion assay with cloxacillin as a control. Pseudomonas spp. was tested with our novel antimicrobial compound. The zones of inhibition in agar culture were measured for each individual component and for the compound, and the results were compared with those of the control group which had been treated with cloxacillin. Data were expressed as means ${\pm}$ standard deviations. Quantitative analyses were performed using the paired t-test. Results: The antibiotic effect of the Phenol-rich compound sweet gel was statistically shown to be more significant than that of cloxacillin against Pseudomonas aeruginosa (P < 0.05). Conclusion: Our novel approach to fighting the antibiotic resistance of Pseudomonas proved to be successful. The Phenol-rich compound sweet gel was found to be suitable for use as an alternative medicine and bioactive dressing material, for the treatment of patients with various types of wounds, including burns, venous leg ulcers, ulcers of various etiologies, leg ulcers on the feet of diabetic, unhealed graft sampling sites, abscesses, boils, surgical wounds, necrotic process, post-operative and neonatal wound infection, and should be considered as an alternative to the usual methods of cure.

• Korean Journal of Plant Resources
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• v.33 no.6
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• pp.578-585
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• 2020

This study was designed to assess the possibility of using medicinal plant extracts as β-lactamase inhibitors to control antibiotic-resistant Staphylococcus aureus. The susceptibilities of S. aureus ATCC 15564 (SA^WT), ciprofloxacininduced S. aureus ATCC 15564 (SA^CIP), oxacillin-induced S. aureus ATCC 15564 (SA^OXA), and clinically-isolated S. aureus CCARM 3008 (SA^CLI) to ampicillin were determined in the absence and presence of medicinal plant extracts, including Cleyera japonica (CJ), Carpinus laxiflora (CL), Euphorbia helioscopia (EH), Euscaphis japonica (EJ), Oenothera erythrosepala (OE), and Rosa multiflora (RM). The phenotypic change in the clear inhibition zones around ampicillin disc was observed for SA^WT, SA^CIP, and SA^OXA, indicating the production of ampicillinase. Compared to the controls, the MICs of ampicillin against SA^WT, SA^CIP, and SA^OXA were decreased from 4 to 0.5 ㎍/mL in the presence of CL, 16 to 4 ㎍/mL in the presence of RM, and 32 to 2 ㎍/mL in the presence of CL, EH, and RM, respectively. The medicinal plant extracts, OE, EJ, and CL, effectively inhibited the β-lactamase activities of SA^WT (78%), SA^CIP (57%), and SA^OXA (76%) when compared to the control. This results suggest that the medicinal plant extracts can be used as BLIs to control the antibiotic-resistant S. aureus.

• The Korea Journal of Herbology
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• v.30 no.5
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• pp.51-58
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• 2015

Objectives : This paper aimed to verify the applicability of mixed extract ofAngelica gigasNakai,Cnidium officinaleMakino,Paeoniala ctifloraPall,Rechmannia glutinosaLibosch,Scutellaria baicalensisGeorgi, which were prescribed for improving inflammation in Donguibogam, as the materials for beauty food and functional medicinal herb cosmetics by manufacturing such mixed extract and evaluating the biological activity of the extract.Methods : The mixed medicinal herb water extract(MMW) and ethanol extract(MME) were freeze-dried to be used as the specimen. We performed electron donating ability, lipid acidification inhibitory activity, anti-inflammatory activity against skin flora, MTT assay, NO inhibitory activity and the protein expression inhibitory activity of iNOS and COX-2.Results : For anti-oxidation experimentation, the electron donating abilities of MMW and MME were above 60.0% and 90.0% at 500 μg/ml, respectively. In the inhibition rate of lipid peroxidation, MMW and MME showed 43.1% and 52.1% at 1,000 μg/ml, respectively. As a result of antimicrobial activity, both the MMW and MME showed significant clear zones forPropionibacterium acnesat 4 mg/disc, but did not indicated the clearzones forStaphylococcus aureus, Escherichia coliandStaphylococcus epidermidis. Anti-inflammatory activity by NO assay showed LPS-induced NO was significantly inhibited in a concentration-dependent manner. Also, the expression of iNOS and COX-2 proteins were significantly inhibited following treatment with MMW and MME of 50 μg/ml.Conclusions : Accordingly, it can be concluded that mixed medicinal herb extract has the potential to beused as a functional food and cosmetic material.