• Title/Summary/Keyword: zinc-dependent

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Dissolution and Characteristics of Antheraea pernyi Silk Fibroin Regenerated from Zinc Chloride Solution (염화아연에 의한 작잠견피브로인의 용해와 특성)

  • 권해용;이광길;우순옥;박영환
    • Journal of Sericultural and Entomological Science
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    • v.44 no.2
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    • pp.87-92
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    • 2002
  • Dissolution of Antheraea pernyi silk fiber was carried out in a zinc chloride solution with various dissolving conditions. The solubility was significantly dependent on the concentration of zinc chloride, dissolving temperature and time. The proper conditions of dissolution were found as 8 M zinc chloride, 70$^{\circ}C$ temperature and 30 min dissolving time. Regenerated A. pernyi silk fibroin powder was obtained through dialysis. FTIR and XRD showed that regenerated A. pernyi silk powder was composed of a ${\beta}$-sheet as well as an ${\alpha}$-helix conformation.

Protective Effects of Dohongsamul-tang on Zinc-mediated Cytotoxicity in H9c2 Cardiomyoblast Cells (산화적 손상에 의해 유발된 심근세포 독성에 대한 도홍사물탕의 방어효과)

  • You Bong Sun;Jung Jae Eun;Park Jin Young;Yun Jong Min;Lee In;Moon Byung Soon
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.18 no.5
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    • pp.1374-1381
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    • 2004
  • The water extract of Dohongsamul-tang(DHSMT)has been traditionally used for treatment of ischemic heart in oriental medicine. However, little is known about the mechanism by which the water extract of DHSMT rescues cells from these damages. Therefore, this study was designed to evaluate the protective effects of DHSMT on zinc-mediated cytotoxicity in H9c2 cardiomyoblast cells. This study demonstrates that treatment of H9c2 cells with zinc caused a decrease in cell viability in a dose dependent manner and a chromatin condensation. Zinc induced the cleavage of poly(ADP-ribose) polymerase (PARP). In addition, zinc induced the decrease of Bcl-2, as well as increase of Bak expression and mitochondrial dysfunction. Zinc-induced H9c2 cell death was remarkably prevented by the pretreatment of DHSMT with consistent suppression of the cleavage of poly(ADP-ribose) polymerase (PARP), mitochondrial dysfunction and the expression of Bak and Bcl-2. Taken together, the results suggest that zinc induced severe cell death in H9c2 cardiomyoblast cells via intracellular GSH(reduced glutathione) depletion and the protective effects of DHSMT against oxidative injuries may be achieved through modulation of mitochondrial dysfunction and scavenging of ROS(reactive oxygen species).

Cloning of a matrix metalloproteinase cDNA from Scylliorhinus torazame (두툽상어 matrix metalloproteinase 유전자 cDNA의 클로닝)

  • Kim, Jon Won;Cho, Won Jin;Chun, Kwang Ho;Kim, Kyu-Won;Kim, Yung-Jin;Lee, Sang-Jun;Shin, Hae-Ja;Lim, Woon Ki
    • Journal of Life Science
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    • v.8 no.3
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    • pp.235-240
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    • 1998
  • Matrix metalloproteinases(MMPs) are a group of zinc enzymes responsible for degradation of the matrix components such as collagen and proteoglycans in normal embryogenesis and remodeling and in many disease processes such as arthritis, cancer, periodontitis, and osteprocess. Genetically distince MMPs have been characterized and their genes have been cloned thus far from a variaty of species but not from fishes. In this stydy, a mmp cDNA was cloned by using RT-PCR(reverse transcriptase dependent polymerase chain reaction) from Scylliorhinus toraxzame(shark), agroup of cartilaginous fish, abundant in the coast of Pusan, Korea. It has 74% base homologue with membrane type matrix matalloproteinase-3 genes(mt3-mmps) from human, rat and chick, and also shows more than 90% residue homologue with them. In addition, it has cysteine switch domain, zinc binding domain(HExGH motif), propeptide cleavage site, and RRKR motif, which are present in MMPs. This result indicates that cDNA fragment cloned here may be mt3-mmp or its analogous gejne cDNA fragment of Scylliorhinus torzame.

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Electrical and Optical Properties of Zinc Oxide Thin Films Deposited Using Atomic Layer Deposition

  • Kim, Jeong-Eun;Bae, Seung-Muk;Yang, Hee-Sun;Hwang, Jin-Ha
    • Journal of the Korean Ceramic Society
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    • v.47 no.4
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    • pp.353-356
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    • 2010
  • Zinc oxide (ZnO) thin films were deposited using atomic layer deposition. The electrical and optical properties were characterized using Hall measurements, spectroscopic ellipsometry and UV-visible spectrophotometry. The electronic concentration and the mobility were found to be critically dependent on the deposition temperature, exhibiting increased resistivity and reduced electronic mobility at low temperature. The corresponding optical properties were measured as a function of photon energy ranging from 1.5 to 5.0 eV. The simulated extinction coefficients allowed the determination of optical band gaps, i.e., ranging from 3.36 to 3.41 eV. The electronic carrier concentration appears to be related to the reduction in the corresponding band gap in ZnO thin films.

Effect of Ultrasonic Wave on the Nickel-Zine Alloy Deposition whit the Variation of Ammonium Chloride Concertration (Ni-Zn 함금도금에서 염화암몬 농도에 따른 초음파의 영향)

  • 양학희;고광진;김재원
    • Journal of Surface Science and Engineering
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    • v.21 no.4
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    • pp.168-175
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    • 1988
  • The nickel0zinc allot depositions have been studjen in ammonium chloridw added chloride baths to fine out the effects of ultrasonic irradiation for the electrodeposition processes. The compositions of deposited alloys, the current efficiencies, corrosion resistance and brightness in various conditions of electrodeposition were investigated, in the range of ultrasonic irradiation of 50,500 and 1,000kc/s respectively. The results obtained are as follows; 1. the ratio of nikel to zinc in the deposit increased according 시 the ammonium chloride concentration in irradiated baths. 2. The current efficiencies became also higer in the irradiated bath. 3. Ammonium ions in solution seem to retard formation of zinc hydroxide. 4. The corrsion resistance and brightness of the deposits are dependent upon nickel content of deposits which ranges 10-18%(wt)nickel in the irradiated baths and 11-15%(wt)in ninirradisted baths. 5. The corrosion resistance and brightnes of the deposited are appreciably better in the irradiated baths than in non-irradiated bath with the mole ratio of 3.4(NH+4/Ni+++Zn++).

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Effects of Nucleating Seeds on Coloring of Zn2SiO4 Crystal Glazes

  • Lee, Hyun-Soo
    • Journal of the Korean Ceramic Society
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    • v.52 no.3
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    • pp.197-203
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    • 2015
  • The colorization of $Zn_2SiO_4$ crystal glazes was investigated by adding nucleating seeds with various coloring agents. The addition of color fixing agents such as $Fe_2O_3$, $MnO_2$, and NiO with seeds caused changes in the colors of glazes. The crystallinity and crystal size were dependent on glaze composition and firing schedules. By controlling coloring agents and firing schedules, it was possible to create various colors and sizes of crystals in a zinc-based crystalline glaze.

Liquid Phase Deposition of Transition Metal Ferrite Thin Films: Synthesis and Magnetic Properties

  • Caruntu Gabriel;O'Connor Charles J.
    • Journal of the Korean Ceramic Society
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    • v.43 no.11 s.294
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    • pp.703-709
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    • 2006
  • We report on the synthesis of highly uniform, single phase zinc and cobalt thin films prepared by the Liquid Phase Deposition (LPD) method. X-Ray diffraction, TGA and EDX measurements support the assumption that the as deposited films are constituted by a mixture of crystallized FeOOH and amorphous M(OH)$_2$ (M=Co, Zn) which is converted upon heat treatment in air at 600?C into the corresponding zinc ferrites. The films with adjustable chemical compositions are identified with a crystal structure as spinel-type and present a spherical or rod-like microstructure, depending on the both the nature and concentration of the divalent transition metal ions. Zinc ferrite thin films present a superparamagnetic behavior above blocking temperatures which decrease with increasing the Zn content and are ferromagnetic at 5 K with coercivities ranging between 797.8 and 948.5 Oe, whereas the cobalt ferrite films are ferromagnetic at room temperature with magnetic characteristics strongly dependent on the chemical composition.

A Study on the Cytotoxic Effect of Heavy metals (Cd, Ni, Zn) on Cultured Mouse Fibroblast L929 Cell line (생쥐 배양섬유 모세포주 L929에 미치는 중금속(Cd, Ni, Zn)류의 세포독성에 관한 연구)

  • 이종빈;나명석;황영진;위성욱;최진희;김선희;유춘만;김재민
    • Journal of Environmental Health Sciences
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    • v.23 no.2
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    • pp.98-105
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    • 1997
  • The study on the cytotoxicity of heavy metals was carried out to evaluate the cytotoxic effect of those on mouse L929 fibroblast cell in 96-well microtiter plates. The cytotoxicity was assayed by the neutral red, tetrazolium MTT, total protein, micronuclei test. The cytotoxicity of the heavy metals by neutral red and tetrazolium MTT was showed in order, cadmium > zinc > nickel for the cationic metals tested. The effect of metal-metal interaction on the cytotoxicity showed a marked reduction of cadmium toxicity by zinc, to a lesser degree, by nickel. The amount of total protein in treated group added heavy metals was less than that of the control and treated cadmium alone was less than those of combination with nickel or zinc. At midpoint cytotoxicity values of heavy metals, the frequency of micronuclei on the cell treated heavy metals was more than that of control and treated cadmium alone was more than those of combination with nickel or zinc. From those results, it could be suggested that the heavy metals decreased the viability of mouse fibroblast L929 cells in a concentration-dependent manner and have cytogenic toxic effects, but mixed group decreased the cytotoxic and cytogenic toxicity on L929 cells.

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Temperature Dependent Behavior of Thermal and Electrical Contacts during Resistance Spot Welding

  • Kim, E.
    • International Journal of Korean Welding Society
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    • v.2 no.1
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    • pp.1-10
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    • 2002
  • The thermal contact conductance at different temperatures and with different electrode forces and zinc coating morphology was measured by monitoring the infrared emissions from the one dimensionally simulated contact heat transfer experiments. The contact heat transfer coefficients were presented as a function of the harmonic mean temperature of the two contacting surfaces. Using these contact heat transfer coefficients and experimentally measured temperature profiles, the electrical contact resistivities both for the faying interface and electrode-workpiece interface were deduced from the numerical analyses of the one dimension simulation welding. It was found that the average value of the contact heat transfer coefficients for the material with zinc coating (coating weight from 0 g/$mm^2$to 100 g/$mm^2$) ranges from 0.05 W/$mm^2$$^{\circ}C$ to 2.0 W/$mm^2$$^{\circ}C$ in the temperature range above 5$0^{\circ}C$ harmonic mean temperature of the two contacting surfaces. The electrical contact resistivity deduced from the one dimension simulation welding and numerical analyses showed that the ratio of electrical contact resistivity at the laying interface to the electrical contact resistivity at the electrode interface is smaller than one far both bare steel and zinc coated steel.

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Nd:YVO4 Laser Patterning of Various Transparent Conductive Oxide Thin Films on Glass Substrate at a Wavelength of 1,064 nm

  • Wang, Jian-Xun;Kwon, Sang Jik;Cho, Eou Sik
    • Transactions on Electrical and Electronic Materials
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    • v.14 no.2
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    • pp.59-62
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    • 2013
  • At an infra-red (IR) wavelength of 1,064 nm, a diode-pumped Q-switched $Nd:YVO_4$ laser was used for the direct patterning of various transparent conductive oxide (TCO) thin films on glass substrate. With various laser beam conditions, the laser ablation results showed that the indium tin oxide (ITO) film was removed completely. In contrast, zinc oxide (ZnO) film was not etched for any laser beam conditions and indium gallium zinc oxide (IGZO) was only ablated with a low scanning speed. The difference in laser ablation is thought to be due to the crystal structures and the coefficient of thermal expansion (CTE) of ITO, IGZO, and ZnO. The width of the laser-patterned grooves was dependent on the film materials, the repetition rate, and the scanning speed of the laser beam.