• Asian-Australasian Journal of Animal Sciences
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• v.6 no.1
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• pp.107-113
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• 1993

This study determined the concentrations of micro-minerals in pastures, in feed supplements and in grazing, reproducing ewes, at different times during the year, at three farms in Northern China. Samples were collected 5 to 8 times during the year and analysed for selenium, copper, iron, molybdenum and zinc. On two farms selenium concentrations in both pastures and animal tissues were low for part of the year. The lowest concentrations in pasture (< $30{\mu}g/kg$ DM) and liver (< $100{\mu}g/kg$ wet weight) indicated that productivity of the sheep may be reduced by a deficiency of this element. On one farm copper concentrations in the lever were in the liver were in the deficient range (< $5{\mu}g/kg$ wet weight) for part of the year. It is likely that this is a result of high intakes of iron from pasture (up to 4.5 g Fe/kg DM) and soil, as indicated by high concentrations of iron in faeces (up to 7 g Fe/kg DM). Molybdenum intake is unlikely to have had much influence on copper absorption because pasture concentrations of this element were not unusually high (1 to 5 mg/kg DM). Zinc in pastures on two farms was below 10 mg/kg DM for part of the year. On one of these farms, the concentration of zinc in faeces was below 30 mg/kg DM throughout the year and this is consistent with zinc intakes of 7 to 15 mg/kg. Despite these low intakes, the concentratons of zinc in plasma were consistently above deficient levels. No clinical signs of deficiencies of any of the elements studied were observed.

• Clinical and Experimental Pediatrics
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• v.48 no.4
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• pp.406-410
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• 2005

Purpose : Anticonvulsants have a number of side effects and some of them may be attributed to a disturbance of serum trace metal homeostasis. Although they are minor building components in tissues, they play important functional roles in the peripheral and central nervous system. We measured serum copper and zinc levels in epileptic children who were treated with anticonvulsants to know the effects of anticonvulsants on serum copper and zinc levels. Methods : Serum copper and zinc levels were determined in 64 epileptic patients receiving anticonvulsant therapy in Chungnam National University Hospital, and in 20 normal controls. Sixty-four epileptic patients were divided into three groups : 16 patients who were treated with valproic acid monotherapy; 26 patients who were treated with valproic acid in addition to other anticonvulsants; and 22 patients who were treated with anticonvulsants except for valporic acid. Results : All patients receiving anticonvulsants had significantly lower serum copper levels($80.21{\pm}19.42{\mu}g/dL$) in comparison to the normal controls($102.12{\pm}32.8{\mu}g/dL$). Serum zinc levels in patients receiving anticonvulsants($79.78{\pm}21.88{\mu}g/dL$) were not statistically different from those of controls ($85.26{\pm}29.81{\mu}g/dL$). There were no significant difference of serum copper and zinc levels among the three groups. Conclusion : In this study, we clearly showed that anticonvulsants decreased serum copper levels. Although we did not observe any clinical findings related to copper deficiency, we should pay attention to potent copper deficiency in patients with anticonvulsant treatment.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.4
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• pp.519-528
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• 2003

A survey was conducted in certain parts of Haryana to record the prevalence of micro mineral deficiency in buffaloes. The prevalence of soil Cu, Co, Zn and Fe deficiency was 55.26%, 6.9%, 59.12% and 7.89% respectively. While that of fodder Cu, Co, Zn and Fe was 60.64%, 6.7%, 61.22% and 11.37% respectively. The overall prevalence of serum Cu, Co, Zn and Fe deficiency in Haryana was 59.2%, 19.1%, 59.2% and 19.9% respectively. The correlation co-efficient of Cu, Co, Zn and Fe in soil, fodder and serum was significant in most of the cases the values were above 0.8. Blood examination revealed significant decrease in haemoglobin and TEC level. However, no variation in level of TLC were observed in mineral deficient buffaloes. In micro mineral deficiency, thyroid hormone (T3 and T4) levels were decreased in buffaloes. Marginally lower concentration of vitamin A and E were observed in mineral deficient buffaloes in Haryana. The highest deficiency of micro minerals was 61.76% in copper at Ambala followed by 65.86% in zinc at Rhotak. For therapeutic studies a mineral mixture was prepared according to defiency obtained and fed to three groups of animals. Observation was recorded on 0, 15, 30, 45 and 60 days. Group A consist of normal healthy animals and group B mineral deficient animal untreated and group C mineral deficient animal, treated with prepared mineral mixture. 25 gram of mineral mixture was fed daily along with normal ration. There was increase in body weight, milk yield, haemoglobin concentration and total erythrocyte level in group C animals when compared to group B animals. The milk yield in group C animals increased to 6.970${\pm}$0.41 after 60th day of supplement in comparison to 0 day where it was 5.910${\pm}$0.37, similarly the body wt. of group C animals increased from 129.42${\pm}$01.13 (at 0 day) to 159.31${\pm}$03.61 at 60th day of treatment.

• Journal of Nutrition and Health
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• v.41 no.8
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• pp.691-700
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• 2008

One suggested mechanism underlying copper (Cu) deficiency teratogenicity is a low availability of nitric oxide (NO), signaling molecule which is essential in developmental processes. Increased superoxide anions secondary to decreased activities of Cu-zinc superoxide dismutase (Cu-Zn SOD) in Cu deficiency can interact with NO to form peroxynitrite, which can nitrate proteins at tyrosine residues. In addition, peroxynitrite formation can limit NO bioavailability. We previously reported low NO availability and increased protein nitration in Cu deficient (Cu-) embryos. In the current study, we tested whether Cu deficiency alters downstream signaling of NO by assessing cyclic GMP (cGMP) and phosphorylated vasodilator-stimulating phosphoprotein (VASP) levels, and whether NO supplementation can affect these targets as well as protein nitration. Gestation day 8.5 embryos from Cu adequate (Cu+) or Cu- dams were collected and cultured in either Cu+ or Cu- media for 48 hr. A subset of embryos was cultured in Cu- media supplemented with a NO donor (DETA/NONOate; 20 ${\mu}M$) and/or Cu-Zn SOD. Cu-/Cu- embryos showed a higher incidence of embryonic and yolk sac abnormalities, low NO availability, blunted dose-response in NO concentrations to increasing doses of acetylcholine, low mRNA expression of endothelial nitric oxide synthase (eNOS), increased levels of 3-nitrotyrosine (3-NT) compared to Cu+/Cu+ controls. cGMP concentrations tended to be low in Cu-/Cu- embryos, and they were significantly lower in Cu-/Cu- yolk sacs than in controls. Levels of phosphorylated VASP at serine 239 (P-VASP) were similar in all groups. NO donor supplementation to the Cu- media ameliorated embryonic and yolk sac abnormalities, and resulted in increased levels of cGMP without altering levels of P-VASP and 3-NT. Taken together, these data support the concept that Cu deficiency limits NO availability and alters NO/cGMP-dependent signaling in Cu- embryos and yolk sacs, which contributes to Cu deficiency-induced abnormal development.

• Korean Journal of Soil Science and Fertilizer
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• v.7 no.1
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• pp.43-47
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• 1974

Arsenic toxicity of rice and the interaction between As and Zn were studied in a greenhouse experiment. The symptom of As toxicity of rice was the growth retardation of tops and roots accompanied by wilting. The toxicity was observed even on the 1 ppm arsenic treated soils. Zinc applictiaon reduced As toxicity but it did not depress the As uptake by rice, while As reduced the Zn uptake by rice. There-fore, the applied Zn apparantly conpensated for the depressed Zn up-take due to As toxicity. Iron deficiency was observed during the experiment, and this was probably due to the relatively high soil reaction, pH of the Crowley silt loam.

• Korean Journal of Community Nutrition
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• v.12 no.1
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• pp.68-79
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• 2007

Iron deficiency is the most common nutritional problem that affects people of all ages in both industrialized and developing countries. Especially, college women are the target population for dietary iron deficiency. Recent study showed that the nutritional status of college women was poor because of insufficient food consumption and repeatabled weight reduction. The purpose of this study was to investigate the effects of nutrition counseling on the diet quality, nutritional status of iron and hematic parameters in college women who have self-recognized anemic symptoms. Anthropometric and dietary assessments as well as blood analysis, were carried out before and after the 8 weeks of nutrition counseling. During the experimental period, the 31 subjects were given nutrition education by a clinical dietitian. Nutrition counseling consisted of pathology of anemia, nutrition information for iron deficient anemia, diet information of balanced meals and menu choices for eating out. As a result, the frequencies of consumption of legumen and vegetables significantly increased after nutrition counseling(p<0.05). Daily intakes of protein, especially animal protein, vitamin E, niacin, heme iron, and zinc significantly increased(p<0.05). After nutrition counseling, some self-reported clinical symptoms such as 'dizziness', 'fatigue', 'short of breath', 'headache', 'sleeplessness', and 'beating heart' were significantly improved. Serum levels of transferrin(p<0.01) and total iron binding capacity levels(p<0.05) significantly increased. It could be concluded that the 8 weeks of nutrition counseling might be effective on quality of diet as well as iron status and it might also improve the some hematic parameters in college women who have self-recognized anemic symptoms.

• Asian-Australasian Journal of Animal Sciences
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• v.1 no.2
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• pp.73-79
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• 1988

Experiments were conducted to determine whether supplemental cobalt (Co) or nickel (Ni) would prevent the signs of zinc (Zn) deficiency in chicks fed a high calcium (1.5%) corn-soybean diet and to examine the difference in $^{65}Zn$ absorption rates between inbred Ancona and crossbred New Hampshire X Single Comb White Leghorn chicks. In the initial experiment, the supplementation of 27 ppm Ni, 27 ppm Co or 54 ppm Co to a basal diet increased weight gain and reduced feather defects; 54 ppm Ni tended to increase weight gain but did not reduce feather defects. In further experiments, chicks fed the diet supplemented with 54 ppm Co usually showed increased weight gain and reduced feather defects; however, chicks fed a diet supplemented with 54 ppm Ni less frequently showed these effects. In another test, Ancona chicks fed a diet supplemented with 30 ppm Zn (except during a $^{65}Zn$ absorption study period) showed lower weight gain, more feather defects and less $^{65}Zn$ absorption than did New Hampshire X Leghorn cross chicks. Similar results were achieved with two strains of chicks fed the basal and 54 ppm Ni, 54 ppm Co or 60 ppm Zn supplemented diets. The sparing effects of Co on Zn which were commonly observed and the lesser effect of Ni or Zn were shown to be, at least in part, the result of increased availability of dietary Zn. That Ancona chicks required more Zn than New Hampshire cross chicks for the development of feathers and for growth is partly the result of decreased Zn absorption from the type of diets fed.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.2 no.2
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• pp.233-239
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• 1999

Acrodermatitis enteropethica (AE) is a rare autosomal recessive disorder of zinc absorption leading to chronic diarrhea and characteristic skin lesion. The term is also applied to any acquired zinc deficiency state resulting in the same clinical pictures. We experienced one case of AE in 1 month old male infant who had bacterial enterocolitis. The skin around mouth, anus, eyes, ears, hands and legs became reddish, vesicular and eczematoid. Serum zinc level was decreased to $51.4\;{\mu}g/dL$ (N=70~150). Endoscopic finding revealed pale gastric mucosa and villous atrophy of small intestine. Biopsy finding of small intestine showed no villi due to mucosal atrophy. On 13 day of admission jaundice with DIC were noted and AST & ALT were elevated to 110 & 36.8 IU/L, respectively. Diarrhea was improved but jaundice and liver function were not recovered until discharge from hospital. After discharge when the patient was 4 months of age serum bilirubin and AST/ALT had not been normalized. CMV shell vial culture of urine and CMV Ig G antibody were positive. So intravenous ganciclovir injection of 7.5 mg/kg, two times a day for 2 weeks and then 10 mg/kg/day for 3 months was done from 4 to 6 months of age. No virus was found in the urine and AST & ALT were normalized at 2 months after stopping ganciclovir treatment.

• Nutrition Research and Practice
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• v.13 no.1
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• pp.41-46
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• 2019

BACKGROUND/OBJECTIVES: Taste perception is influenced by both nutritional factors and psychological factors. This study was undertaken to measure the 4 basic taste perceptions, nutrient intake, and mental health, and to examine the factors that affect insensitive taste perception in young Japanese women. SUBJECTS/METHODS: Young women in their late teens and twenties were enrolled as subjects. Taste perception was measured by applying the filter-paper disc method over areas of the chorda tympani nerve. Nutritional status was evaluated using brief, self-administered diet history questionnaires. The index of nutritional status was based on the 2015 Japanese dietary reference intakes. Mental health was assessed using the Japanese translation of the Profile of Mood States short version. This study was approved by the ethical committee at Osaka University. RESULTS: The normal taste perception group (four basic tastes [sweet, salty, sour, and bitter] identified as normal taste perception) comprised 55.4% of the subjects, while the abnormal taste perception group (more than 1 abnormal taste perception was perceived, regardless of flavor) comprised 44.6% of the subjects. There were no significant differences in nutrient intake (except manganese) and mental health between the normal and abnormal taste perception groups. Subjects who took 5 mg to less than 7.1 mg zinc per day were at significantly decreased risk of insensitive taste perception compared to subjects who consumed less than 5 mg zinc per day [Regression coefficient 0.831, 95% confidence interval 0.694-0.996]. CONCLUSION: The present results suggest that insensitive taste perception could be associated with zinc deficiency in young women in their late teens and twenties.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.9 no.1
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• pp.14-22
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• 2006

Purpose: To identify factors that influence serum zinc concentrations in children with acute gastroenteritis. Methods: Thirty-two children under 5 years of age (15 boys and 17 girls) were selected randomly among those who visited to an pediatric emergency room of Ehwa Womans University Mokdong Hospital with acute gastroenteritis from May to August 2005. This study estimated the association between serum zinc concentrations and clinical, biochemical variables in patients with acute gastroenteritis. Results: Serum zinc concentration was lower in febrile patients than afebrile patients with acute gastroenteritis ($67.5{\pm}25.3$ vs $85.5{\pm}14.2$, p<0.05). It also was lower in patients with positive C-reactive protein (CRP) than those with negative CRP ($63.9{\pm}25.4$vs $86.7{\pm}13.8$, p<0.05). Serum zinc concentration was negatively correlated (r=-0.494, p<0.05) with CRP concentration, whereas positively correlated with hematocrit (r=0.370, p<0.05), total protein (r=0.474, p<0.05), and albumin (r=0.636, p<0.05). Twelve patients (37.5%) showed very low serum zinc concentration (< $70{\mu}g/dL$) without clinical symptoms of deficiency or growth retardation. Frequency of febrile illness or positive CRP is significantly greater in group with zinc < $70{\mu}g/dL$ than the group with zinc ${\geq}70{\mu}g/dL$ (91.7% vs 55%, p<0.05; 91.7% vs 40%, p<0.05, respectively). Conclusion: In patients with acute gastroenteritis, serum zinc concentration was influenced by various factors such as fever, CRP, and biochemical factors. For evaluating zinc status in the body. factors.